https://scholars.lib.ntu.edu.tw/handle/123456789/456363
標題: | Transforming growth factor alpha promotes osteosarcoma metastasis by ICAM-1 and PI3K/Akt signaling pathway | 作者: | CHUN-HAN HOU Lin F.-L. Tong K.-B. Hou S.-M. Liu J.-F. |
公開日期: | 2014 | 出版社: | Elsevier Inc. | 卷: | 89 | 期: | 4 | 起(迄)頁: | 453-463 | 來源出版物: | Biochemical Pharmacology | 摘要: | Osteosarcoma is the most common primary malignancy of bone and is characterized by a high malignant and metastatic potential. Transforming growth factor alpha (TGF-α) is classified as the EGF (epidermal growth factor)-like family, which is involved in cancer cellular activities such as proliferation, motility, migration, adhesion and invasion abilities. However, the effect of TGF-α on human osteosarcoma is largely unknown. We found that TGF-α increased the cell migration and expression of intercellular adhesion molecule-1 (ICAM-1) in human osteosarcoma cells. Transfection of cells with ICAM-1 siRNA reduced TGF-α-mediated cell migration. We also found that the phosphatidylinositol 3′-kinase (PI3K)/Akt/NF-κB pathway was activated after TGF-α treatment, and TGF-α-induced expression of ICAM-1 and cell migration was inhibited by the specific inhibitors and siRNAs of PI3K, Akt, and NF-κB cascades. In addition, knockdown of TGF-α expression markedly decreased cell metastasis in vitro and in vivo. Our results indicate that TGF-α/EGFR interaction elicits PI3K and Akt activation, which in turn activates NF-κB, resulting in the expression of ICAM-1 and contributing the migration of human osteosarcoma cells. ? 2014 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901607271&doi=10.1016%2fj.bcp.2014.03.010&partnerID=40&md5=30dd313604d5dbdeb6415e3f2be4a50d https://scholars.lib.ntu.edu.tw/handle/123456789/456363 |
ISSN: | 0006-2952 | DOI: | 10.1016/j.bcp.2014.03.010 | SDG/關鍵字: | intercellular adhesion molecule 1; phosphatidylinositol 3 kinase; protein kinase B; transforming growth factor alpha; animal experiment; article; cell migration; human; human cell; human cell culture; in vitro study; in vivo study; male; metastasis; mouse; nonhuman; osteosarcoma; osteosarcoma cell line; priority journal; protein expression; signal transduction; tumor xenograft; Migration; Osteosarcoma; TGF-α; Animals; Antineoplastic Agents; Bone Neoplasms; Cell Line, Tumor; Cell Movement; Humans; Intercellular Adhesion Molecule-1; Lung Neoplasms; Male; Mice; Mice, SCID; NF-kappa B; Osteosarcoma; Phosphatidylinositol 3-Kinase; Proto-Oncogene Proteins c-akt; Receptor, Epidermal Growth Factor; Recombinant Proteins; RNA Interference; Second Messenger Systems; Transforming Growth Factor alpha; Tumor Burden; Up-Regulation; Xenograft Model Antitumor Assays |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。