https://scholars.lib.ntu.edu.tw/handle/123456789/456898
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chang W.-F. | en_US |
dc.contributor.author | Wu Y.-H. | en_US |
dc.contributor.author | Xu J. | en_US |
dc.contributor.author | Sung L.-Y. | en_US |
dc.contributor.author | LI-YING SUNG | zz |
dc.creator | Chang W.-F.;Wu Y.-H.;Xu J.;Sung L.-Y. | - |
dc.date.accessioned | 2020-02-10T09:00:07Z | - |
dc.date.available | 2020-02-10T09:00:07Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 16616596 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/456898 | - |
dc.description.abstract | Mammalian telomere lengths are primarily regulated by telomerase, consisting of a reverse transcriptase protein (TERT) and an RNA subunit (TERC). We previously reported the generation of mouse Terc+/? and Terc?/? embryonic stem cells (ntESCs) by somatic cell nuclear transfer. In the present work, we investigated the germ layer development competence of Terc?/?, Terc+/? and wild-type (Terc+/+) ntESCs. The telomere lengths are longest in wild-type but shortest in Terc?/? ntESCs, and correlate reversely with the population doubling time. Interestingly, while in vitro embryoid body (EB) differentiation assay reveals EB size difference among ntESCs of different genotypes, the more stringent in vivo teratoma assay demonstrates that Terc?/? ntESCs are severely defective in differentiating into the mesodermal lineage cartilage. Consistently, in a directed in vitro chondrocyte differentiation assay, the Terc?/? cells failed in forming Collagen II expressing cells. These findings underscore the significance in maintaining proper telomere lengths in stem cells and their derivatives for regenerative medicine. ? 2019, MDPI AG. All rights reserved. | - |
dc.relation.ispartof | International Journal of Molecular Sciences | - |
dc.subject | Embryonic stem cells; Mesoderm; Mouse; Telomerase; Telomeres | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | collagen type 2; hand 1 protein; octamer transcription factor 4; Sal like protein 4; telomerase; testis determining factor; transcription factor GATA 4; transcription factor NANOG; transcription factor Sox17; transcription factor Sox2; transcription factor Sox9; unclassified drug; RNA; telomerase RNA; animal cell; animal experiment; animal model; animal tissue; Article; cell differentiation; cell nucleus transplantation; chondrocyte; confocal microscopy; controlled study; embryo; embryonic stem cell; enzyme linked immunosorbent assay; fibroblast; flow cytometry; gene expression; gene frequency; germ layer; human; human cell; immunofluorescence; immunohistochemistry; mouse; nonhuman; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; somatic cell; telomere length; Western blotting; animal; C57BL mouse; cartilage; cell culture; cell nucleus; cell nucleus transplantation; chondrocyte; chondrogenesis; genetics; HEK293 cell line; knockout mouse; mouse embryonic stem cell; physiology; telomere; telomere homeostasis; Animals; Cartilage; Cell Differentiation; Cell Nucleus; Cells, Cultured; Chondrocytes; Chondrogenesis; HEK293 Cells; Humans; Mice; Mice, Inbred C57BL; Mice, Knockout; Mouse Embryonic Stem Cells; Nuclear Transfer Techniques; RNA; Telomerase; Telomere; Telomere Homeostasis | - |
dc.title | Compromised chondrocyte differentiation capacity in terc knockout mouse embryonic stem cells derived by somatic cell nuclear transfer | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/ijms20051236 | - |
dc.identifier.scopus | 2-s2.0-85063002287 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85063002287&doi=10.3390%2fijms20051236&partnerID=40&md5=11518a4c23ba06f29819cbbeb1f89824 | - |
dc.relation.journalvolume | 20 | - |
dc.relation.journalissue | 5 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.orcid | 0000-0003-1221-2476 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
顯示於: | 生物科技研究所 |
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