https://scholars.lib.ntu.edu.tw/handle/123456789/457019
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chen K.-I. | en_US |
dc.contributor.author | Pan C.-Y. | en_US |
dc.contributor.author | Li K.-H. | en_US |
dc.contributor.author | Huang Y.-C. | en_US |
dc.contributor.author | Lu C.-W. | en_US |
dc.contributor.author | Tang C.-Y. | en_US |
dc.contributor.author | Su Y.-W. | en_US |
dc.contributor.author | Tseng L.-W. | en_US |
dc.contributor.author | Tseng K.-C. | en_US |
dc.contributor.author | Lin C.-Y. | en_US |
dc.contributor.author | Chen C.-D. | en_US |
dc.contributor.author | Lin S.-S. | en_US |
dc.contributor.author | YIT-TSONG CHEN | en_US |
dc.contributor.author | SHIH-SHUN LIN | en_US |
dc.contributor.author | CHIEN-YUAN PAN | en_US |
dc.creator | Chen K.-I.;Pan C.-Y.;Li K.-H.;Huang Y.-C.;Lu C.-W.;Tang C.-Y.;Su Y.-W.;Tseng L.-W.;Tseng K.-C.;Lin C.-Y.;Chen C.-D.;Lin S.-S.;Chen Y.-T. | - |
dc.date.accessioned | 2020-02-10T09:00:43Z | - |
dc.date.available | 2020-02-10T09:00:43Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 20452322 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/457019 | - |
dc.description.abstract | Many transcribed RNAs are non-coding RNAs, including microRNAs (miRNAs), which bind to complementary sequences on messenger RNAs to regulate the translation efficacy. Therefore, identifying the miRNAs expressed in cells/organisms aids in understanding genetic control in cells/organisms. In this report, we determined the binding of oligonucleotides to a receptor-modified silicon nanowire field-effect transistor (SiNW-FET) by monitoring the changes in conductance of the SiNW-FET. We first modified a SiNW-FET with a DNA probe to directly and selectively detect the complementary miRNA in cell lysates. This SiNW-FET device has 7-fold higher sensitivity than reverse transcription-quantitative polymerase chain reaction in detecting the corresponding miRNA. Next, we anchored viral p19 proteins, which bind the double-strand small RNAs (ds-sRNAs), on the SiNW-FET. By perfusing the device with synthesized ds-sRNAs of different pairing statuses, the dissociation constants revealed that the nucleotides at the 3′-overhangs and pairings at the terminus are important for the interactions. After perfusing the total RNA mixture extracted from Nicotiana benthamiana across the device, this device could enrich the ds-sRNAs for sequence analysis. Finally, this bionanoelectronic SiNW-FET, which is able to isolate and identify the interacting protein-RNA, adds an additional tool in genomic technology for the future study of direct biomolecular interactions. | - |
dc.relation.ispartof | Scientific Reports | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | microRNA; nanowire; silicon; chemistry; conformation; devices; gene expression profiling; gene silencing; genetics; nanotechnology; procedures; RNA interference; RNA processing; transistor; Gene Expression Profiling; Gene Silencing; MicroRNAs; Nanotechnology; Nanowires; Nucleic Acid Conformation; RNA Interference; RNA Processing, Post-Transcriptional; Silicon; Transistors, Electronic | - |
dc.title | Isolation and identification of post-transcriptional gene silencing-related micro-RNAs by functionalized silicon nanowire field-effect transistor | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/srep17375 | - |
dc.identifier.scopus | 2-s2.0-84948659700 | - |
dc.identifier.url | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84948659700&doi=10.1038%2fsrep17375&partnerID=40&md5=7316b3478c8ccfeba8cc3d74f57c2466 | - |
dc.relation.journalvolume | 5 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Chemistry | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Neurobiology and Cognitive Science Center | - |
crisitem.author.orcid | 0000-0002-6204-8320 | - |
crisitem.author.orcid | 0000-0002-7295-5004 | - |
crisitem.author.orcid | 0000-0002-6654-9309 | - |
crisitem.author.parentorg | College of Science | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
Appears in Collections: | 生物科技研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.