https://scholars.lib.ntu.edu.tw/handle/123456789/457219
標題: | The critical role of ECM proteins within the human MSC niche in endothelial differentiation | 作者: | Wang, Chih-Hsiang TING-MING WANG Tai-Horng Young Lai, Yiu-Kay MEN-LUH YEN |
關鍵字: | Bone;Endothelial differentiation;Extracellular matrix;FOXC2;Integrin αvβ3/CD61;Mesenchymal stem cells | 公開日期: | 六月-2013 | 卷: | 34 | 期: | 17 | 起(迄)頁: | 4223 | 來源出版物: | Biomaterials | 摘要: | Interactions between blood vessels and osteoblasts-bone-forming cells-are critical for successful bone development. We therefore investigated the endothelial differentiation capacity of mesenchymal stem cells (MSCs) derived from bone tissue. We found that fetal pre-osteoblast and adult trabecular bone-derived (TB) MSCs express similar surface markers as bone marrow (BM) MSCs and can differentiate into adipocytes, osteoblasts, and chondrocytes. However, when cultured in extracellular matrix (ECM) and endothelial differentiation conditions, bone-derived MSCs (B-MSCs) more readily form tubular structures and uptake acetylated low-density lipoproteins, fulfilling the functional criteria for endothelial cells (ECs). Moreover, addition of B-MSCs but not other cells significantly enhanced vessel formation in the in vivo chick chorioallantoic membrane assay. Mechanistically, this appears to be due to the upregulation of the endothelial transcription factor forkhead box protein C2 (FOXC2) and its downstream gene αvβ3 integrin/CD61in B-MSCs but not BMMSCs by laminin, a component protein of the ECM. Our findings not only reveal discrepant differentiation capacity for various tissue-specific MSCs, but also highlight the critical role of the niche-in this case, the ECM and its component proteins-in determining lineage commitment of stem cells. © 2013 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/457219 | ISSN: | 0142-9612 | DOI: | 10.1016/j.biomaterials.2013.02.062 |
顯示於: | 醫學系 |
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