|Title:||Upregulation of Focal adhesion kinase by 14-3-3ε via NFκB activation in Hepatocellular Carcinoma||Authors:||BOR-SHENG KO
|Issue Date:||2013||Journal Volume:||13||Journal Issue:||4||Start page/Pages:||555-562||Source:||Anti-Cancer Agents in Medicinal Chemistry||Abstract:||
Focal adhesion kinase (FAK) is implicated in cancer cell survival, proliferation and migration. Expression of FAK expression is elevated and associated with tumor progression and metastasis in various tumors, including hepatocellular carcinoma (HCC). Increased 14-3-3ε expression is shown to be a potential prognostic factor to predict higher risk of distant metastasis and worse overall survival in HCC. The aim of this study is to investigate whether FAK is associated or regulated by 14-3-3ε to modulate tumor progression in HCC. In this study, 114 primary HCC tumors including 34 matched metastatic tumors were subjected to immunohistochemistry analysis of FAK and 14-3-3ε expression. Overexpression of FAK was significantly associated with increased risk of extrahepatic metastasis (p=0.027) and reduced 5-year overall survival rate (p=0.017). A significant correlation of FAK and 14-3-3ε expression was observed in primary tumor (p<0.001) and also metastatic tumors. Furthermore, overexpression of 14-3-3ε induced FAK expression and promoter activity which were determined by Western blotting analysis and luciferase-reporter assay. Moreover, 14-3-3ε enhanced NFκB activation and increased nuclear translocation of NFκB. Results from chromatin immunoprecipitation assay revealed that 14-3-3ε induced NFκB binding on FAK promoter region. These findings suggest that FAK expression is correlated with and upregulated by 14-3-3ε via activation of NFκB. Target to suppress or inactivate FAK alone, or combine with 14-3-3ε is thus considered as the potential therapeutic strategy for preventing HCC tumor progression. ? 2013 Bentham Science Publishers.
|ISSN:||1871-5206||DOI:||10.2174/1871520611313040004||SDG/Keyword:||focal adhesion kinase; immunoglobulin enhancer binding protein; protein 14 3 3; adult; aged; article; cancer risk; cell fractionation; chromatin immunoprecipitation; controlled study; female; gene overexpression; human; human cell; human tissue; immunohistochemistry; liver cell carcinoma; liver metastasis; major clinical study; male; overall survival; promoter region; protein expression; tumor growth; upregulation; Western blotting; 14-3-3 Proteins; Antineoplastic Agents; Boronic Acids; Carcinoma, Hepatocellular; Cell Survival; Drug Screening Assays, Antitumor; Female; Focal Adhesion Protein-Tyrosine Kinases; Humans; Kaplan-Meier Estimate; Liver Neoplasms; Male; Middle Aged; NF-kappa B; Pyrazines; Structure-Activity Relationship; Tumor Cells, Cultured; Up-Regulation
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.