https://scholars.lib.ntu.edu.tw/handle/123456789/457870
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Liu T.-A. | en_US |
dc.contributor.author | Jan Y.-J. | en_US |
dc.contributor.author | BOR-SHENG KO | en_US |
dc.contributor.author | Liang S.-M. | en_US |
dc.contributor.author | Chen S.-C. | en_US |
dc.contributor.author | Wang J. | en_US |
dc.contributor.author | CHIUN HSU | en_US |
dc.contributor.author | YAO-MING WU | en_US |
dc.contributor.author | Liou J.-Y. | en_US |
dc.creator | Liu T.-A.;Jan Y.-J.;Ko B.-S.;Liang S.-M.;Chen S.-C.;Wang J.;Hsu C.;Yao-Ming Wu;Liou J.-Y. | - |
dc.date.accessioned | 2020-02-11T07:58:43Z | - |
dc.date.available | 2020-02-11T07:58:43Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874599208&doi=10.1371%2fjournal.pone.0057968&partnerID=40&md5=d93f1c06b6cb08cf295190e77e3b7379 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/457870 | - |
dc.description.abstract | Background: 14-3-3ε is implicated in regulating tumor progression, including hepatocellular carcinoma (HCC). Our earlier study indicated that elevated 14-3-3ε expression is significantly associated with higher risk of metastasis and lower survival rates of HCC patients. However, the molecular mechanisms of how 14-3-3ε regulates HCC tumor metastasis are still unclear. Methodology and Principal Findings: In this study, we show that increased 14-3-3ε expression induces HCC cell migration and promotes epithelial-mesenchymal transition (EMT), which is determined by the reduction of E-cadherin expression and induction of N-cadherin and vimentin expression. Knockdown with specific siRNA abolished 14-3-3ε-induced cell migration and EMT. Furthermore, 14-3-3ε selectively induced Zeb-1 and Snail expression, and 14-3-3ε-induced cell migration was abrogated by Zeb-1 or Snail siRNA. In addition, the effect of 14-3-3ε-reduced E-cadherin was specifically restored by Zeb-1 siRNA. Positive 14-3-3ε expression was significantly correlated with negative E-cadherin expression, as determined by immunohistochemistry analysis in HCC tumors. Analysis of 14-3-3ε/E-cadherin expression associated with clinicopathological characteristics revealed that the combination of positive 14-3-3ε and negative E-cadherin expression is significantly correlated with higher incidence of HCC metastasis and poor 5-year overall survival. In contrast, patients with positive 14-3-3ε and positive E-cadherin expression had better prognostic outcomes than did those with negative E-cadherin expression. Significance: Our findings show for the first time that E-cadherin is one of the downstream targets of 14-3-3ε in modulating HCC tumor progression. Thus, 14-3-3ε may act as an important regulator in modulating tumor metastasis by promoting EMT as well as cell migration, and it may serve as a novel prognostic biomarker or therapeutic target for HCC. ? 2013 Liu et al. | - |
dc.relation.ispartof | PLoS ONE | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | nerve cell adhesion molecule; protein 14 3 3; protein 14 3 3 epsilon; small interfering RNA; transcription factor Snail; transcription factor ZEB1; unclassified drug; uvomorulin; vimentin; cadherin; homeodomain protein; protein 14 3 3; snail family transcription factors; transcription factor; YWHAE protein, human; ZEB1 protein, human; article; cancer cell; cancer growth; cancer incidence; cancer patient; cancer prognosis; cancer survival; cell migration; clinical feature; controlled study; correlation analysis; enzyme activity; epithelial mesenchymal transition; human; human cell; human tissue; liver cell carcinoma; major clinical study; metastasis; outcome assessment; protein expression; protein function; cell motion; gene expression regulation; genetics; Kaplan Meier method; liver tumor; metabolism; middle aged; pathology; prognosis; tumor cell line; upregulation; 14-3-3 Proteins; Cadherins; Carcinoma, Hepatocellular; Cell Line, Tumor; Cell Movement; Epithelial-Mesenchymal Transition; Gene Expression Regulation, Neoplastic; Homeodomain Proteins; Humans; Kaplan-Meier Estimate; Liver Neoplasms; Middle Aged; Neoplasm Metastasis; Prognosis; Transcription Factors; Up-Regulation | - |
dc.title | 14-3-3ε Overexpression Contributes to Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/journal.pone.0057968 | - |
dc.identifier.pmid | 23483955 | - |
dc.identifier.scopus | 2-s2.0-84874599208 | - |
dc.relation.journalvolume | 8 | - |
dc.relation.journalissue | 3 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Medical Oncology-NTUCC | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.orcid | 0000-0002-7965-7579 | - |
crisitem.author.orcid | 0000-0002-1122-0055 | - |
crisitem.author.orcid | 0000-0002-1720-7863 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
顯示於: | 醫學系 |
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