https://scholars.lib.ntu.edu.tw/handle/123456789/458252
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chou C.-H | en_US |
dc.contributor.author | LING-HUNG WEI | en_US |
dc.contributor.author | Kuo M.-L | en_US |
dc.contributor.author | RUBY YUN-JU HUANG | en_US |
dc.contributor.author | Lai K.-P | en_US |
dc.contributor.author | CHI-AN CHEN | en_US |
dc.contributor.author | CHANG-YAO HSIEH | en_US |
dc.creator | Hsieh C.-Y.;Chen C.-A;Lai K.-P;Huang Y.-J;Kuo M.-L;LING-HUNG WEI;Chou C.-H | - |
dc.date.accessioned | 2020-02-12T05:48:33Z | - |
dc.date.available | 2020-02-12T05:48:33Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/458252 | - |
dc.description.abstract | Bioactive lysophospholipid, lysophosphatidic acid (LPA), is consistently raised in the ascites of patients with ovarian cancer. Interleukin-6 (IL-6) is a pleiotropic cytokine, which is assumed to be involved in ovarian carcinogenesis. However, the regulation of IL-6 in ovarian cancer remains largely unknown. To elucidate the pathogenesis of ovarian cancer, this study investigated how LPA affects IL-6 production in ovarian cancer cells. Experimental results indicated that LPA stimulates IL-6 expression in all ovarian cancer cell lines tested, but not in normal ovarian surface epithelial (NOSE) cells, owing to the lack of LPA-specific Edg4 and/or Edg7 receptors in NOSE cells. This work demonstrated that LPA transcriptionally activates IL-6 expression, which can be totally blocked by the pertussis toxin, indicating that Gi-mediated signaling is critically involved in inducing IL-6 by LPA. Pharmacological and genetic inhibition assays revealed that Gi-mediated PI3K activation phosphorylated downstream Akt and subsequently induced NF-κB activation causes the induction of IL-6 by LPA in SK-OV-3 cells. In summary, data presented here demonstrate that LPA is an important inducer of IL-6 and LPA-regulated IL-6 expression via a Gi/PI3K-Akt/NF-κB pathway in ovarian cancer cells, providing molecular therapeutic targets for treating ovarian cancer. ? Oxford University Press 2005; all rights reserved. | en_US |
dc.relation.ispartof | Carcinogenesis | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | 2 (2 amino 3 methoxyphenyl)chromone; 2 morpholino 8 phenylchromone; G protein coupled receptor; immunoglobulin enhancer binding protein; interleukin 6; lysophosphatidic acid; pertussis toxin; phosphatidylinositol 3 kinase; protein kinase B; article; cancer cell culture; carcinogenesis; controlled study; cytokine production; enzyme activation; enzyme immunoassay; enzyme phosphorylation; female; gel mobility shift assay; gene expression; genetic transcription; human; human cell; ovary cancer; priority journal; reverse transcription polymerase chain reaction; statistical analysis; statistical significance; Western blotting; 1-Phosphatidylinositol 3-Kinase; Blotting, Western; Cell Line, Tumor; Electrophoretic Mobility Shift Assay; Enzyme Activation; Female; Heterotrimeric GTP-Binding Proteins; Humans; Interleukin-6; Lysophospholipids; NF-kappa B; Ovarian Neoplasms; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-akt; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Up-Regulation | - |
dc.title | Up-regulation of interleukin-6 in human ovarian cancer cell via a Gi/PI3K-Akt/NF-κB pathway by lysophosphatidic acid, an ovarian cancer-activating factor | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1093/carcin/bgh301 | - |
dc.identifier.pmid | 15471896 | - |
dc.identifier.scopus | 2-s2.0-13444302907 | - |
dc.relation.pages | 45-52 | en_US |
dc.relation.journalvolume | 26 | en_US |
dc.relation.journalissue | 1 | en_US |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | School of Medicine | - |
crisitem.author.dept | Master's Program in Smart Medicine and Health Informatics (SMARTMHI) | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.orcid | 0000-0001-8789-0859 | - |
crisitem.author.orcid | 0000-0001-6376-3185 | - |
crisitem.author.orcid | 0000-0001-6670-7939 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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