https://scholars.lib.ntu.edu.tw/handle/123456789/458280
標題: | Divergent endometrial inflammatory cytokine expression at peri-implantation period and after the stimulation by copper intrauterine device | 作者: | Chou C.-H SHEE-UAN CHEN CHIA-TUNG SHUN PO-NIEN TSAO YU-SHIH YANG JEHN-HSIAHN YANG |
公開日期: | 2015 | 卷: | 5 | 起(迄)頁: | 15157 | 來源出版物: | Scientific Reports | 摘要: | Endometrial inflammation has contradictory effects. The one occurring at peri-implantation period is favourable for embryo implantation, whereas the other occurring after the stimulation by copper intrauterine device (Cu-IUD) prevents from embryo implantation. In this study, 8 week female ICR mice were used to investigate the endometrial inflammation, in which they were at proestrus stage (Group 1), at peri-implantation period (Group 2), and had a copper wire implanted into right uterine horn (Group 3). Cytokine array revealed that two cytokines were highly expressed in Group 2 and Group 3 as compared with Group 1, and seven cytokines, including tumour necrosis factor α (TNF-α), had selectively strong expression in Group 3. Immunohistochemistry demonstrated prominent TNF-α staining on the endometrium after Cu-IUD stimulation, and in vitro culture of human endometrial glandular cells with Cu induced TNF-α secretion. The increased TNF-α concentration enhanced in vitro THP-1 cells chemotaxis, and reduced embryo implantation rates. These results suggest that inflammatory cytokine profiles of endometrium are different between those at peri-implantation period and after Cu-IUD stimulation, and TNF-α is the one with selectively strong expression in the latter. It might account for the contradictory biological effects of endometrial inflammation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/458280 | ISSN: | 2045-2322 | DOI: | 10.1038/srep15157 | SDG/關鍵字: | copper; cytokine; tumor necrosis factor; animal; cell culture; chemistry; chemotaxis; copper intrauterine device; cytology; drug effects; endometrium; female; human; immunohistochemistry; inflammation; Institute for Cancer Research mouse; metabolism; mouse; nidation; protein microarray; secretion (process); Animals; Cells, Cultured; Chemotaxis; Copper; Cytokines; Embryo Implantation; Endometrium; Female; Humans; Immunohistochemistry; Inflammation; Intrauterine Devices, Copper; Mice; Mice, Inbred ICR; Protein Array Analysis; Tumor Necrosis Factor-alpha |
顯示於: | 醫學系 |
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