https://scholars.lib.ntu.edu.tw/handle/123456789/458431
標題: | Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS | 作者: | CHU-CHUN HUANG Chou C.-H SHEE-UAN CHEN HONG-NERNG HO YU-SHIH YANG MEI-JOU CHEN |
公開日期: | 2019 | 卷: | 118 | 期: | 1P2 | 起(迄)頁: | 249-259 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background/Purpose: Abnormal folliculogenesis is one of the cardinal presentations of polycystic ovarian syndrome (PCOS) and permeability of follicular wall has been proposed to be involved in the normal follicular growth. However, whether or not there is a change in intrafollicular permeability underlies PCOS is unknown. Methods: This was a tertiary center-based case–control study. From 2014 to 2015, thirteen patients with PCOS who underwent in vitro fertilization-embryo transfer (IVF-ET) were enrolled. Eleven normo-ovulatory patients who underwent IVF-ET due to male factor and/or tubal factor infertility were enrolled as the control group. The influence of ovarian follicular fluid (FF) on endothelial cell permeability was evaluated using a human umbilical vein endothelial cell monolayer permeability assay. The intrafollicular expression profiles of angiogenesis-related proteins were analyzed using a Human Angiogenesis Protein Array Kit. Results: The FF from PCOS patients caused significantly poorer endothelial cell permeability comparing with the effect of FF from the control group (46% ± 12% vs. 58% ± 9%, P = 0.023). Among the 55 angiogenesis-related proteins tested, there was a significantly higher level of intrafollicular platelet factor 4 (PF4) and PF4/IL-8 complex in the PCOS group (p = 0.004). The anti-permeability effect of PF4 was related to the decrease in the intercellular gaps and antagonistic binding with IL-8. Conclusion: Our study provides the first evidence of the pathophysiologic contribution of the well-known angiostatic protein, PF4, on human reproductive biology. The increase of the intrafollicular PF4 and its anti-permeability effect might affect the formation of FF and folliculogenesis in PCOS. ? 2018 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/458431 | ISSN: | 0929-6646 | DOI: | 10.1016/j.jfma.2018.05.002 | SDG/關鍵字: | amphiregulin; angiogenin; angiopoietin 2; cetrorelix; CXCL16 chemokine; cysteine rich protein 61; estradiol; gelatinase B; human menopausal gonadotropin; interleukin 8; keratinocyte growth factor; leptin; luteinizing hormone; pentraxin 3; progesterone; prolactin; recombinant chorionic gonadotropin; recombinant follitropin; thrombocyte factor 4; thrombospondin 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 4; triptorelin; vasculotropin; thrombocyte factor 4; adult; angiogenesis; Article; blood vessel permeability; case control study; cell membrane permeability; comparative study; controlled study; embryo transfer; estradiol blood level; female; human; human cell; hyperandrogenism; in vitro fertilization; luteinizing hormone blood level; metaphase; oligomenorrhea; oocyte maturation; oocyte retrieval; ovarian reserve; ovary follicle fluid; ovary polycystic disease; pathophysiology; photometry; progesterone blood level; protein expression; tertiary care center; umbilical vein endothelial cell; Western blotting; chemistry; female infertility; ovary follicle fluid; ovary polycystic disease; pathology; permeability; Taiwan; Adult; Case-Control Studies; Female; Fertilization in Vitro; Follicular Fluid; Humans; Infertility, Female; Permeability; Platelet Factor 4; Polycystic Ovary Syndrome; Taiwan; Tertiary Care Centers |
顯示於: | 醫學系 |
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