https://scholars.lib.ntu.edu.tw/handle/123456789/458528
標題: | Fluorescence of carbazole derivatives for screening of human cancer | 作者: | Tseng T.-Y. Chen W.-W. Chu I.-T. Wang C.-L. Chang C.-C. MEI-CHUN LIN PEI-JEN LOU Chang T.-C. |
關鍵字: | cancer diagnosis; carbazole derivatives; FLIM; Fluorescent probe; G-quadruplex; o-BMVC foci | 公開日期: | 2019 | 卷: | 10853 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | Bioimaging of fluorescent probes provides a fantastic tool to visualize different cellular responses from various cells and to monitor target/probe interaction in cells. Previously, we introduced a small molecule named 3,6-bis(1-methyl-4- vinylpyridinium)carbazole diiodide (BMVC) as a fluorescent marker to light up live cancer cells for cancer diagnosis. Here we used time-gated fluorescence lifetime imaging microscopy (FLIM) of a BMVC isomer, 3,6-bis(1-methyl-2- vinylpyridinium)carbazole diiodide (o-BMVC) as a fluorescent probe to distinguish fixed cancer cells for cancer diagnosis. The time-gated FLIM results showed many more number of o-BMVC foci, characterized by the longer fluorescent decay time of o-BMVC (?2.4 ns), in fixed cancer cells (HeLa, H1299, CL1-0, MCF-7, MCF-7/ADR, and SAS) than in fixed normal cells (MRC-5, IMR-90, and BJ). Further study of tissue biopsy, a total of 50 head and neck cancer (HNC) samples obtained during surgery and 20 normal oral samples collected from healthy volunteers showed that o-BMVC foci are hardly detectable in the normal oral epithelial cells. The average numbers of o-BMVC foci in tumors and in normal oral epithelial cells are 28.3 and 2.2, respectively. The receiver operating characteristic curve analysis showed the area under curve was 0.992, indicating that this method provides a very high accuracy for clinical detection of HNC cancers. We propose that the combination of BMVC test for live cells and o-BMVC test for fixed cells could provide a powerful tool for screening of human cancers. Copyright ? 2019 SPIE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/458528 | ISSN: | 1605-7422 | DOI: | 10.1117/12.2509410 | SDG/關鍵字: | Cytology; Diagnosis; Diseases; Fluorescence imaging; Fluorescence spectroscopy; Imaging techniques; Optical image storage; Polycyclic aromatic hydrocarbons; Probes; Surgery; Cancer diagnosis; Carbazole Derivatives; FLIM; Fluorescent probes; G-quadruplexes; Cells |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。