https://scholars.lib.ntu.edu.tw/handle/123456789/458612
標題: | Integration of human papillomavirus correlates with high levels of viral oncogene transcripts in cervical carcinogenesis | 作者: | Ho C.-M. Lee B.-H. Chang S.-F. Chien T.-Y. Huang S.-H. Yan C.-C. WEN-FANG CHENG |
關鍵字: | Cervical cancer; Human papillomavirus; Integration; Physical state | 公開日期: | 2011 | 卷: | 161 | 期: | 2 | 起(迄)頁: | 124-130 | 來源出版物: | Virus Research | 摘要: | A prospective, cross-sectional study was conducted to investigate the correlation between the integration of high-risk human papillomavirus and disease severity of cervical lesions. 720 liquid-based cytology specimens including 422 normal cytology, 78 low-grade squamous intraepithelial lesions, 172 high-grade squamous intraepithelial lesions, and 48 women with cervical cancers were examined using HPV blot and type-specific E6 PCR. Positive HPV DNA types 16, 18, 52 and 58 were examined for viral DNA using real-time PCR. Expression of E6 transcripts was 89.5% (pure integration), 71.7% (mixed type), and 47.1% (pure episomal) (p< 0.0001). Geometric mean levels ranged from 110.6 (episomal form) to 508.4 (mixed form), and 5966.2 (integration form) by real-time PCR (p< 0.0001). Geometric mean levels of E6 transcript in HPV 16, 18, 52, and 58 correlated with the severity of cervical lesions and the physical integration state of the viral genome (p< 0.0001). We conclude that this is the first paper to point out that integration of high-risk HPVs not only 16 and 18 but also 52 and 58 is correlated with high levels of oncogene transcripts from normal cervix, CIN to cervical cancer. ? 2011 Elsevier B.V. |
URI: | 2-s2.0-80053133401 https://scholars.lib.ntu.edu.tw/handle/123456789/458612 |
ISSN: | 0168-1702 | DOI: | 10.1016/j.virusres.2011.06.012 | SDG/關鍵字: | protein E6; virus DNA; adult; article; carcinogenesis; controlled study; cross-sectional study; disease severity; electrosurgery; female; gene expression; genetic transcription; high grade squamous intraepithelial lesion; human; Human papillomavirus type 16; Human papillomavirus type 18; Human papillomavirus type 52; Human papillomavirus type 58; human tissue; low grade squamous intraepithelial lesion; major clinical study; priority journal; prospective study; real time polymerase chain reaction; uterine cervix cancer; uterine cervix cytology; virus DNA cell DNA interaction; virus genome; virus oncogene; Wart virus; Adult; Alphapapillomavirus; Cervix Uteri; Cross-Sectional Studies; Female; Gene Expression Regulation, Viral; Humans; Middle Aged; Oncogene Proteins, Viral; Papillomavirus Infections; Prospective Studies; Transcription, Genetic; Uterine Cervical Neoplasms; Virus Integration; Human papillomavirus; Human papillomavirus type 16 |
顯示於: | 醫學系 |
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