https://scholars.lib.ntu.edu.tw/handle/123456789/459812
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | SONG-NAN CHOW | en_US |
dc.contributor.author | RUEY-JIEN CHEN | en_US |
dc.contributor.author | CHI-HAU CHEN | en_US |
dc.contributor.author | TING-CHEN CHANG | en_US |
dc.contributor.author | Chen L.C | en_US |
dc.contributor.author | Lee W.J | en_US |
dc.contributor.author | Shen J | en_US |
dc.contributor.author | LU-PING CHOW | en_US |
dc.creator | Chow S.N;Chen R.J;Chen C.H;Ting-Chen Chang;Chen L.C;Lee W.J;Shen J;Chow L.P. | - |
dc.date.accessioned | 2020-02-17T05:28:13Z | - |
dc.date.available | 2020-02-17T05:28:13Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0392-2936 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/459812 | - |
dc.description.abstract | Background: Screening in ovarian cancer is progressively finding out candidate genes and proteins which may work as screening biomarkers and play a role in tumor progression. We examined the protein expression patterns of ovarian cancer tissues using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of fight mass spectrometry (MALDI-TOF MS). Methods: Tissues from 36 ovarian cancers and 20 normal ovaries were examined by 2-DE. The images of silver stained gels were analyzed by ImageMaster 2D Elite. The peptide mixtures, after in-gel digestion, were determined by MALDI-TOF MS for fingerprinting. The de-isotope tryptic peptide profiles were matched by using the Mascot search engine based on the entire NCBI and Swiss-Prot protein databases. Western/dot blots were then applied to verify the findings. Results: In ovarian cancer, 12 proteins that showed differential expressions were identified unequivocally. Among these proteins, five proteins (galectin-1, cathepsin B, ubiquitin carboxy-terminal hydrolase L1, HLA class II antigen DRB1-11 and heat shock protein 27) were up-regulated and seven proteins (cellular retinol-binding protein, transthyretin, SH3 binding glutamic-rich-like protein, tubulin-specific chaperone A, DJ-1, gamma-actin and tropomyosin 4) were down-regulated. Conclusion: The present study is the first to report the up-regulation of ubiquitin carboxy-terminal hydrolase L1 and the down-regulation of SH3 binding glutamic-rich-like protein, tubulin-specific chaperone A, and tropomyosin 4 in human ovarian cancer tissues. Further cloning and functional analysis of these salient proteins will provide more information on their pathophysiologic roles in ovarian cancer. | en_US |
dc.relation.ispartof | European Journal of Gynaecological Oncology | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cathepsin B; cellular retinol binding protein; DJ 1 protein; galectin 1; gamma actin; heat shock protein 27; HLA antigen class 2; prealbumin; protein; SH3 binding glutamic rich like protein; tropomyosin 4; tubulin specific chaperone A; ubiquitin thiolesterase; unclassified drug; amino acid sequence; article; cancer tissue; controlled study; down regulation; female; finger dermatoglyphics; human; human tissue; matrix assisted laser desorption ionization time of flight mass spectrometry; ovary cancer; polyacrylamide gel electrophoresis; protein database; protein expression; proteomics; search engine; two dimensional gel electrophoresis; upregulation; Western blotting; Adult; Aged; Aged, 80 and over; Blotting, Western; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Female; Humans; Middle Aged; Neoplasm Proteins; Ovarian Neoplasms; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.title | Analysis of protein profiles in human epithelial ovarian cancer tissues by proteomic technology | en_US |
dc.type | journal article | en |
dc.identifier.pmid | 20349782 | - |
dc.identifier.scopus | 2-s2.0-77349101807 | - |
dc.relation.pages | 55-62 | en_US |
dc.relation.journalvolume | 31 | en_US |
dc.relation.journalissue | 1 | en_US |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHJS | - |
crisitem.author.dept | Biochemistry and Molecular Biology | - |
crisitem.author.orcid | 0000-0003-0291-006X | - |
crisitem.author.orcid | 0000-0003-4903-7878 | - |
crisitem.author.orcid | 0000-0003-3867-3801 | - |
crisitem.author.orcid | 0000-0002-4420-211X | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Jin-shan Branch | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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