https://scholars.lib.ntu.edu.tw/handle/123456789/460298
標題: | Lysophosphatidic acid mediates interleukin-8 expression in human endometrial stromal cells through its receptor and nuclear factor-κB- dependent pathway: A possible role in angiogenesis of endometrium and placenta | 作者: | SHEE-UAN CHEN HSINYU LEE DAW-YUAN CHANG Chou C.-H. Chang C.-Y. KUANG-HAN CHAO CHUNG-WU LIN YU-SHIH YANG |
公開日期: | 2008 | 卷: | 149 | 期: | 11 | 起(迄)頁: | 5888-5896 | 來源出版物: | Endocrinology | 摘要: | Lysophosphatidic acid (LPA) is a pleiotropic phospholipid molecule involved in inflammation, angiogenesis, would healing, and cancer invasion. Whereas serum lysophospholipaseD activity increases in women with pregnancy, the role of LPA in pregnancy remains unclear. We investigated the expression of LPA receptors and function of LPA in endometrial stromal cells. Histologically normal endometrium was obtained from surgical specimens of women undergoing hysterectomy for leiomyoma. First-trimester decidua was obtained from women receiving elective termination of pregnancy. We examined the expressions of LPA1, LPA2, and LPA3 receptors in endometrial stromal cells. The effects of LPA on the expression of vascular endothelial growth factor, IL-6, and IL-8 were examined. Signal pathways of LPA were delineated. Functions of secretory angiogenic factors were tested using human endometrial microvascular endothelial cells. Immunoreactivity and mRNA of LPA1 receptors were identified in endometrial stromal cells. LPA enhanced IL-8 expression in a dose- and time-dependent manner, whereas vascular endothelial growth factor or IL-6 expression was not affected by LPA treatment. Mechanistic dissection disclosed that LPA functioned via the Gi protein, MAPK/p38 and nuclear factor-κB pathway. LPA-induced IL-8 enhanced migration, permeability, capillary tube formation, and proliferation of human endometrial microvascular endothelial cells. Endometrial stromal cells express LPA1 receptors. Through the LPA1 receptor, LPA induces IL-8 expression via a nuclear factor-κB-dependent signal pathway. These results could suggest that LPA may play a role in angiogenesis of endometrium and placenta through induction of IL-8 in endometrial stromal cells during pregnancy. Copyright ? 2008 by The Endocrine Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/460298 | ISSN: | 0013-7227 | DOI: | 10.1210/en.2008-0314 | SDG/關鍵字: | immunoglobulin enhancer binding protein; inhibitory guanine nucleotide binding protein; interleukin 6; interleukin 8; lysophosphatidic acid; lysophosphatidic acid 1 receptor; lysophosphatidic acid 2 receptor; lysophosphatidic acid 3 receptor; lysophosphatidic acid receptor; lysophospholipase; lysophospholipase D; mitogen activated protein kinase p38; unclassified drug; vasculotropin; angiogenesis; article; cell membrane permeability; cell migration; cell proliferation; endometrium; female; human; human cell; human tissue; immunoreactivity; placenta; pregnancy; priority journal; Adult; Cells, Cultured; Endometrium; Female; Gene Expression Regulation; Humans; Interleukin-8; Lysophospholipids; Models, Biological; Neovascularization, Physiologic; NF-kappa B; Placenta; Pregnancy; Receptors, Lysophosphatidic Acid; RNA, Messenger; Signal Transduction; Stromal Cells |
顯示於: | 醫學系 |
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