https://scholars.lib.ntu.edu.tw/handle/123456789/460788
標題: | MicroRNA-17-5p post-transcriptionally regulates p21 expression in irradiated betel quid chewing-related oral squamous cell carcinoma cells | 作者: | Wu S.-Y Lin K.-C Chiou J.-F Jeng S.-C Cheng W.-H Chang C.-L Lin W.-C Wu L.-L Lee H.-L RUEY-JIEN CHEN |
公開日期: | 2013 | 卷: | 189 | 期: | 8 | 起(迄)頁: | 675-683 | 來源出版物: | Strahlentherapie und Onkologie | 摘要: | Background and purpose: Betel nut chewing is associated with oral cavity cancer in Taiwan. OC3 is an oral carcinoma cell line that was established from cells collected from a long-term betel nut chewer who does not smoke. After we found that microRNA-17-5p (miR-17-5p) is induced in OC3 cells, we used this cell line to examine the biological role(s) of this microRNA in response to exposure to ionizing radiation. Materials and methods: A combined SYBR green-based real-time PCR and oligonucleotide ligation assay was used to examine the expression of the miR-17 polycistron in irradiated OC3 cells. The roles of miR-17-5p and p21 were evaluated with specific antisense oligonucleotides (ODN) that were designed and used to inhibit their expression. Expression of the p21 protein was evaluated by Western blotting. The clonogenic assay and annexin V staining were used to evaluate cell survival and apoptosis, respectively. Cells in which miR-17-5p was stably knocked down were used to create ectopic xenografts to evaluate in vivo the role of miR-17-5p. Results: A radiation dose of 5 Gy significantly increased miR-17-5p expression in irradiated OC3 cells. Inhibition of miR-17-5p expression enhanced the radiosensitivity of the OC3 cells. We found that miR-17-5p downregulates radiation-induced p21 expression in OC3 cells and, by using a tumor xenograft model, it was found that p21 plays a critical role in increasing the radiosensitivity of OC3 cells in vitro and in vivo. Conclusion: miR-17-5p is induced in irradiated OC3 cells and it downregulates p21 protein expression, contributing to the radioresistance of OC3 cells. ? 2013 Springer Heidelberg Berlin. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/460788 | ISSN: | 0179-7158 | DOI: | 10.1007/s00066-013-0347-9 | SDG/關鍵字: | antisense oligonucleotide; microRNA; microRNA17 5p; protein p21; unclassified drug; animal experiment; animal model; animal tissue; apoptosis; article; betel nut; cancer radiotherapy; carcinoma cell; cell cycle progression; cell survival; clonogenic assay; colony formation; controlled study; DNA binding; flow cytometry; immunohistochemistry; mastication; mouse; mouth squamous cell carcinoma; nick end labeling; nonhuman; protein expression; radiation dose; radiosensitivity; real time polymerase chain reaction; transcription regulation; tumor xenograft; Western blotting; Administration, Oral; Areca; Carcinoma, Squamous Cell; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; Mouth Neoplasms; Radiation Tolerance; RNA Processing, Post-Transcriptional |
顯示於: | 醫學系 |
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