https://scholars.lib.ntu.edu.tw/handle/123456789/461587
標題: | Expression of Metastatic Tumor Antigen 1 Splice Variant Correlates With Early Recurrence and Aggressive Features of Hepatitis B Virus–Associated Hepatocellular Carcinoma | 作者: | Li Y.-T. Wu H.-L. JIA-HORNG KAO Cheng H.-R. MING-CHIH HO Wang C.-C. PEI-JER CHEN DING-SHINN CHEN CHUN-JEN LIU |
公開日期: | 2019 | 出版社: | John Wiley and Sons Inc. | 卷: | 70 | 期: | 1 | 起(迄)頁: | 184-197 | 來源出版物: | Hepatology | 摘要: | Overexpression of metastatic tumor antigen 1 (MTA1) was correlated with poor prognosis of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HBV-HCC). The aim of this study was to examine the clinical significance of the expression of MTA1 and its exon 4-excluded form (MTA1dE4), the most abundant spliced variant of MTA1, in patients receiving curative resection for HBV-HCC. We collected 102 patients with?HBV-HCC and received curative resection retrospectively and examined the expressions level of total MTA1/MTA1dE4 in their paired nontumor and tumor liver tissues by using RT-qPCR. The association between MTA1/MTA1dE4 expression and various tumor features as well as tumor recurrence was analyzed. During the median follow-up period of 4 years, 25 patients (24.5%) showed early recurrence (within 12 months postresection) and 42 (54.5%) showed late recurrence. In Kaplan-Meier analysis, MTA1dE4 overexpression in tumor, but not MTA1, was associated with early recurrence (P = 0.0365), but not late recurrence. In multivariate analysis, only alpha-fetoprotein (AFP) ?200 ng/mL (P = 0.006) and large tumor size (P = 0.027) were correlated with early recurrence. In the subgroup of patients with AFP <200 ng/mL, high MTA1dE4, but not total MTA1, expression could help predict early recurrence (P = 0.0195). In vitro, wound healing and invasion assays were performed in HCC cells, and MTA1dE4 was found to exhibit a higher ability in promoting migration and invasion of hepatoma cells than full-length MTA1. Conclusion: MTA1dE4 expression is correlated with more aggressive tumor characteristics and might serve as a more sensitive marker for early recurrence of HBV-HCC, especially for low-AFP patients. ? 2019 by the American Association for the Study of Liver Diseases. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064510890&doi=10.1002%2fhep.30581&partnerID=40&md5=148d387c7660092dd010b53d658b37f9 https://scholars.lib.ntu.edu.tw/handle/123456789/461587 |
ISSN: | 0270-9139 | DOI: | 10.1002/hep.30581 | SDG/關鍵字: | alpha fetoprotein; biological marker; lamivudine; metastatic tumor antigen 1; mta1de4 protein; tumor antigen; unclassified drug; isoprotein; Mta1 protein, human; repressor protein; transactivator protein; tumor marker; adult; antigen expression; Article; cancer prognosis; cell invasion; cell migration; chronic hepatitis B; clinical outcome; controlled study; female; gene overexpression; Hepatitis B virus; histopathology; human; human cell; human tissue; in vitro study; liver cell carcinoma; liver resection; liver tissue; major clinical study; male; middle aged; predictive value; priority journal; protein expression level; quantitative analysis; real time polymerase chain reaction; recurrence risk; retrospective study; risk factor; SK-HEP-1 cell line; tumor number; tumor recurrence; tumor volume; wound healing assay; aged; complication; hepatitis B; liver; liver cell carcinoma; liver tumor; metabolism; pathology; tumor recurrence; virology; Adult; Aged; Biomarkers, Tumor; Carcinoma, Hepatocellular; Female; Hepatitis B; Humans; Liver; Liver Neoplasms; Male; Middle Aged; Neoplasm Recurrence, Local; Protein Isoforms; Repressor Proteins; Retrospective Studies; Trans-Activators |
顯示於: | 醫學系 |
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