https://scholars.lib.ntu.edu.tw/handle/123456789/462185
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Huang, Chung-Yen | en_US |
dc.contributor.author | Huang, Ching-Ying | en_US |
dc.contributor.author | Pai, Yu-Chen | en_US |
dc.contributor.author | BEEN-REN LIN | en_US |
dc.contributor.author | TSUNG-CHUN LEE | en_US |
dc.contributor.author | PI-HUI LIANG | en_US |
dc.contributor.author | LINDA CHIA-HUI YU | en_US |
dc.creator | Huang C.-Y.;Huang C.-Y.;Pai Y.-C.;Been-Ren Lin;Lee T.-C.;Liang P.-H.;Yu L.C.-H. | - |
dc.date.accessioned | 2020-02-24T02:29:10Z | - |
dc.date.available | 2020-02-24T02:29:10Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 2234-943X | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076698639&doi=10.3389%2ffonc.2019.01282&partnerID=40&md5=22789a217f9f93660be7279b464a1073 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/462185 | - |
dc.description.abstract | Reprogrammed glucose metabolism and increased glycolysis have been implicated in tumor chemoresistance. The aim was to investigate the distinct roles of the glucose metabolites pyruvate and ATP in chemoresistance mechanisms, including cell death and proliferation. Our data showed higher glucose transporters in colorectal cancer (CRC) from non-responsive patients than those responsive to chemotherapy. Human CRC cell lines exposed to 5-fluorouracil (5-FU) displayed elevated cell viability and larger tumors in xenograft mouse models if cultured in high-glucose medium. Glucose conferred resistance to 5-FU-induced necroptosis via pyruvate scavenging of mitochondrial free radicals, whereas ATP replenishment had no effect on cell death. Glucose attenuated the 5-FU-induced G0/G1 shift but not the S phase arrest. Opposing effects were observed by glucose metabolites; ATP increased while pyruvate decreased the G0/G1 shift. Lastly, 5-FU-induced tumor spheroid destruction was prevented by glucose and pyruvate, but not by ATP. Our finding argues against ATP as the main effector for glucose-mediated chemoresistance and supports a key role of glycolytic pyruvate as an antioxidant for dual modes of action: necroptosis reduction and a cell cycle shift to a quiescent state. ? Copyright ? 2019 Huang, Huang, Pai, Lin, Lee, Liang and Yu. | - |
dc.publisher | Frontiers Media S.A. | - |
dc.relation.ispartof | Frontiers in Oncology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | adenosine triphosphate; antioxidant; fluorouracil; glucose; glucose transporter 1; glucose transporter 3; glucose transporter 4; lactate dehydrogenase; phloretin; phlorizin; pyruvic acid; reactive oxygen metabolite; receptor interacting protein serine threonine kinase; sodium glucose cotransporter 1; animal experiment; animal model; animal tissue; Article; cancer adjuvant therapy; cancer chemotherapy; cancer resistance; cancer staging; cell cycle progression; cell proliferation; cell viability; clinical article; colorectal cancer; controlled study; cytotoxicity; DNA fragmentation; flow cytometry; G1 phase cell cycle checkpoint; gene expression; glucose metabolism; glucose transport; histology; human; human cell; human tissue; IC50; immunoblotting; immunofluorescence; immunoprecipitation; metabolite; MTT assay; necroptosis; nonhuman; protein expression; real time polymerase chain reaction; S phase cell cycle checkpoint; tumor growth; tumor volume; tumor xenograft; Western blotting | - |
dc.title | Glucose Metabolites Exert Opposing Roles in Tumor Chemoresistance | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3389/fonc.2019.01282 | - |
dc.identifier.scopus | 2-s2.0-85076698639 | - |
dc.relation.journalvolume | 9 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Pharmacy | - |
crisitem.author.dept | School of Pharmacy | - |
crisitem.author.dept | Physiology | - |
crisitem.author.orcid | 0000-0001-8354-1758 | - |
crisitem.author.orcid | 0000-0002-8352-3266 | - |
crisitem.author.orcid | 0000-0001-6668-4642 | - |
crisitem.author.orcid | 0000-0003-1461-3249 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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