https://scholars.lib.ntu.edu.tw/handle/123456789/463921
標題: | Screening of a specific peptide binding to esophageal squamous carcinoma cells from phage displayed peptide library | 作者: | Ma, C. Li, C. Jiang, D. Gao, X. Han, J. Xu, N. Wu, Q. Nie, G. Chen, W. Lin, F. Hou, Y. Lin, Feng-Huei |
關鍵字: | Esophageal squamous cell carcinoma; Molecular imaging and targeting of cancer; Peptide; Phage display peptide library | 公開日期: | 2015 | 卷: | 29 | 期: | 3 | 起(迄)頁: | 182-189 | 來源出版物: | Molecular and Cellular Probes | 摘要: | To select a specifically binding peptide for imaging detection of human esophageal squamous cell carcinoma (ESCC), a phage-displayed 12-mer peptide library was used to screen the peptide that bind to ESCC cells specifically. After four rounds of bio-panning, the phage recovery rate gradually increased, and specific phage clones were effectively enriched. The 60 randomly selected phage clones were tested using cellular enzyme-linked immunosorbent assay (ELISA), and 41 phage clones were identified as positive clones with the over 2.10 ratio of absorbance higher than other clones, IRP and PBS controls. From the sequencing results of the positive clones, 14 peptide sequences were obtained and ESCP9 consensus sequence was identified as the peptide with best affinity to ESCC cells via competitive inhibition, fluorescence microscopy, and flow cytometry. The results indicate that the peptide ESCP9 can bind to ESCC cells specifically and sensitively, and it is a potential candidate to be developed as an useful molecule to the imaging detection and targeting therapy for ESCC. ? 2015 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463921 | DOI: | 10.1016/j.mcp.2015.04.001 | SDG/關鍵字: | ABLIM1 protein; aftiphilin protein; CD99L2 protein; cell enzyme; coat protein; DNA directed DNA polymerase alpha; ef hand calcium binding domain containing protein 5; fluorescein isothiocyanate; growth factor receptor bound protein 2; immunoglobulin epsilon heavy chain; mitogen activated protein kinase; peptide; phosphatidylinositol 5 phosphate 4 kinase type 2 alpha; Protein FAM27D1; sodium ascorbic acid cotransporter; transforming, acidic coiled coil containing protein 2; unclassified drug; peptide; peptide library; amino acid sequence; Article; bacteriophage; binding affinity; binding site; cancer screening; cell clone; cell culture; clinical effectiveness; competitive inhibition; consensus sequence; DNA sequence; embryo; enzyme active site; enzyme linked immunosorbent assay; esophageal squamous cell carcinoma; flow cytometry; fluorescence microscopy; HEK293 cell line; human; human cell; image analysis; in vitro study; peptide analysis; peptide library; peptide synthesis; phage display; priority journal; protein binding; protein targeting; screening test; sequence alignment; chemistry; esophagus tumor; metabolism; pathology; peptide library; sensitivity and specificity; squamous cell carcinoma; tumor cell line; Bacteriophages; Carcinoma, Squamous Cell; Cell Line, Tumor; Esophageal Neoplasms; HEK293 Cells; Humans; Peptide Library; Peptides; Sensitivity and Specificity |
顯示於: | 醫學工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。