https://scholars.lib.ntu.edu.tw/handle/123456789/463942
標題: | Application of carbon nanotubes layered on silicon wafer for the detection of breast cancer marker carbohydrate antigen 15-3 by immuno-polymerase chain reaction | 作者: | Sadhasivam, S. Chen, J.-C. Savitha, S. Chang, C.-W. Lin, F.-H. FENG-HUEI LIN |
公開日期: | 2014 | 卷: | 25 | 期: | 1 | 起(迄)頁: | 101-111 | 來源出版物: | Journal of Materials Science: Materials in Medicine | 摘要: | A highly sensitive detection of breast cancer marker, carbohydrate antigen 15-3 (CA 15-3) by carbon nanotube (CNT) based immuno-polymerase chain reaction was reported. The study was aimed to develop a precise and sensitive method to diagnose breast cancer and its recurrence. The hydrofluoric acid (HF) treated silicon wafer layered with bundled CNT was used as the substrate. The surface was treated with HNO3/H2SO4 to graft carboxyl groups on the tips of CNT. Subsequently, polyoxyethylene bis-amine was grafted to conjugate anti human CA 15-3 antibodies. Water contact angle measurement, scanning electron microscope, Fourier transform infrared spectrometer, Raman spectrometer and sodium dodecyl sulfate polyacrylamide gel electrophoresis were employed to confirm the surface modification. The captured antibodies on the CNT were used to capture the target antigen CA 15-3 and the biotinylated secondary antibodies were subsequently bound with the target antigen. A bi-functional streptavidin was used to link biotinylated DNA to the biotinylated detection antibodies. The biotinylated target DNA was amplified by PCR, and then analyzed by agarose gel electrophoresis. The lower limit of detection of CA 15-3 by the proposed immuno-PCR system was 0.001 U/mL, which is extremely sensitive than the other bioanalytical techniques. ? 2013 Springer Science+Business Media New York. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/463942 | DOI: | 10.1007/s10856-013-5060-9 | SDG/關鍵字: | Agarose gel electrophoresis; Bioanalytical techniques; Fourier transform infrared spectrometer; Highly sensitive detections; Immuno-polymerase chain reactions; Lower limit of detections; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Water contact angle measurement; Antibodies; Antigens; Carbohydrates; Carbon nanotubes; Diseases; Electrophoresis; Fourier transform infrared spectroscopy; Grafting (chemical); Hydrofluoric acid; Polyethylene oxides; Scanning electron microscopy; Silicon; Silicon wafers; Sodium dodecyl sulfate; Spectrometers; Polymerase chain reaction; antibody; CA 15-3 antigen; carbon nanotube; DNA; hydrofluoric acid; quantum dot; silicon; streptavidin; tumor marker; water; agar gel electrophoresis; analytic method; analytical equipment; analyzer; article; biotinylation; breast cancer; cancer diagnosis; cancer prognosis; cancer screening; chemical modification; contact angle; diagnostic imaging; DNA binding; DNA sequence; Fourier transformation; human; hydrophilicity; immuno polymerase chain reaction; infrared spectrometry; measurement; morphology; nanotechnology; polyacrylamide gel electrophoresis; polymerase chain reaction; priority journal; protein analysis; qualitative analysis; quantitative analysis; Raman spectrometry; scanning electron microscope; spectrometer; surface property; treatment response; Antibodies, Immobilized; Breast Neoplasms; Female; Humans; Immunoassay; Microscopy, Electron, Scanning; Mucin-1; Nanotubes, Carbon; Polymerase Chain Reaction; Silicon; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; Tumor Markers, Biological |
顯示於: | 醫學工程學研究所 |
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