https://scholars.lib.ntu.edu.tw/handle/123456789/464033
Title: | Chitosan/Gelatin Hydrogel Prolonged the Function of Insulinoma/Agarose Microspheres In Vivo During Xenogenic Transplantation | Authors: | Yang, K.-C. Wu, C.-C. Cheng, Y.-H. Kuo, T.-F. TZONG-FU KUO FENG-HUEI LIN |
Issue Date: | 2008 | Journal Volume: | 40 | Journal Issue: | 10 | Start page/Pages: | 3623-3626 | Source: | Transplantation Proceedings | Abstract: | Purpose: A chitosan/gelatin solution with glycerol 2-phosphate disodium salt hydrate in liquid phase at room temperature becomes a hydrogel at 37°C. The material can be used as an injectable cell carrier into the human body for gelation in situ. We hoped that the chitosan/gelatin hydrogel provided extra protection for insulinoma/agarose microspheres during xenogenic transplantation. Materials and Methods: Mouse insulinoma was microencapsulated in agarose as microspheres, which were macroencapsulated in chitosan/gelatin hydrogel. Insulin secreting profiles were first demonstrated in vitro. Diabetic rats were injected subcutaneously with insulinoma/agarose microspheres or insulinoma/agarose microspheres suspended in chitosan/gelatin solution. The nonfasting blood glucose concentrations (NFBG) of diabetic rats were measured perioperatively. Rats were humanely killed 1 month postoperatively and the hydrogel was retrieved for histological examination. Results: The insulinoma/agarose microspheres continually secreted insulin for 1 month when macroencapsulated in chitosan/gelatin hydrogel in vitro. The NFBG of diabetic rats injected with insulinoma/agarose microspheres decreased to euglycemic status albeit hyperglycemia was restored within 10 days. The NFBG of diabetic rats injected with chitosan/gelatin hydrogel, which contained insulinoma/agarose microspheres, was maintained at less than 200 mg/dL for 25 days. The histological section revealed immune cell infiltration and accumulation within the hydrogel and around the iusulinoma/agarose microspheres that may have contributed to the slowly increasing NFBG after day 25. Conclusion: This study showed that chitosan/gelatin hydrogel can be used as a cell carrier for an injectable bioartificial pancreas; the hydrogel prolonged the function of cells encapsulated in agarose microspheres during xenogenic transplantation. ? 2008 Elsevier Inc. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/464033 | DOI: | 10.1016/j.transproceed.2008.06.092 | SDG/Keyword: | agarose; chitosan; gelatin; glucose; glycerol 2 phosphate; microsphere; animal cell; animal experiment; animal model; animal tissue; article; cell infiltration; concentration (parameters); diabetes mellitus; gelation; glucose blood level; hydrogel; hyperglycemia; immunocompetent cell; in vitro study; in vivo study; insulin release; insulinoma; liquid; microencapsulation; mouse; nonhuman; priority journal; protection; rat; room temperature; xenograft; xenotransplantation; Animals; Chitosan; Gelatin; Hydrogels; Insulin; Insulinoma; Mice; Neoplasm Transplantation; Rats; Sepharose; Transplantation, Heterologous |
Appears in Collections: | 醫學工程學研究所 |
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