https://scholars.lib.ntu.edu.tw/handle/123456789/464033
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Yang, K.-C. | en_US |
dc.contributor.author | Wu, C.-C. | en_US |
dc.contributor.author | Cheng, Y.-H. | en_US |
dc.contributor.author | Kuo, T.-F. | en_US |
dc.contributor.author | TZONG-FU KUO | en_US |
dc.contributor.author | FENG-HUEI LIN | en_US |
dc.creator | Feng-Huei LinYang, K.-C.;Wu, C.-C.;Cheng, Y.-H.;Kuo, T.-F.;Lin, F.-H. | - |
dc.date.accessioned | 2020-02-26T01:33:25Z | - |
dc.date.available | 2020-02-26T01:33:25Z | - |
dc.date.issued | 2008 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/464033 | - |
dc.description.abstract | Purpose: A chitosan/gelatin solution with glycerol 2-phosphate disodium salt hydrate in liquid phase at room temperature becomes a hydrogel at 37°C. The material can be used as an injectable cell carrier into the human body for gelation in situ. We hoped that the chitosan/gelatin hydrogel provided extra protection for insulinoma/agarose microspheres during xenogenic transplantation. Materials and Methods: Mouse insulinoma was microencapsulated in agarose as microspheres, which were macroencapsulated in chitosan/gelatin hydrogel. Insulin secreting profiles were first demonstrated in vitro. Diabetic rats were injected subcutaneously with insulinoma/agarose microspheres or insulinoma/agarose microspheres suspended in chitosan/gelatin solution. The nonfasting blood glucose concentrations (NFBG) of diabetic rats were measured perioperatively. Rats were humanely killed 1 month postoperatively and the hydrogel was retrieved for histological examination. Results: The insulinoma/agarose microspheres continually secreted insulin for 1 month when macroencapsulated in chitosan/gelatin hydrogel in vitro. The NFBG of diabetic rats injected with insulinoma/agarose microspheres decreased to euglycemic status albeit hyperglycemia was restored within 10 days. The NFBG of diabetic rats injected with chitosan/gelatin hydrogel, which contained insulinoma/agarose microspheres, was maintained at less than 200 mg/dL for 25 days. The histological section revealed immune cell infiltration and accumulation within the hydrogel and around the iusulinoma/agarose microspheres that may have contributed to the slowly increasing NFBG after day 25. Conclusion: This study showed that chitosan/gelatin hydrogel can be used as a cell carrier for an injectable bioartificial pancreas; the hydrogel prolonged the function of cells encapsulated in agarose microspheres during xenogenic transplantation. ? 2008 Elsevier Inc. All rights reserved. | - |
dc.relation.ispartof | Transplantation Proceedings | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | agarose; chitosan; gelatin; glucose; glycerol 2 phosphate; microsphere; animal cell; animal experiment; animal model; animal tissue; article; cell infiltration; concentration (parameters); diabetes mellitus; gelation; glucose blood level; hydrogel; hyperglycemia; immunocompetent cell; in vitro study; in vivo study; insulin release; insulinoma; liquid; microencapsulation; mouse; nonhuman; priority journal; protection; rat; room temperature; xenograft; xenotransplantation; Animals; Chitosan; Gelatin; Hydrogels; Insulin; Insulinoma; Mice; Neoplasm Transplantation; Rats; Sepharose; Transplantation, Heterologous | - |
dc.title | Chitosan/Gelatin Hydrogel Prolonged the Function of Insulinoma/Agarose Microspheres In Vivo During Xenogenic Transplantation | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.transproceed.2008.06.092 | - |
dc.identifier.scopus | 2-s2.0-57549116863 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-57549116863&doi=10.1016%2fj.transproceed.2008.06.092&partnerID=40&md5=e2e09f2694b4860d6cdf93038fa87707 | - |
dc.relation.pages | 3623-3626 | - |
dc.relation.journalvolume | 40 | - |
dc.relation.journalissue | 10 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.dept | Biomedical Engineering | - |
crisitem.author.orcid | 0000-0002-6328-8967 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 醫學工程學研究所 |
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