https://scholars.lib.ntu.edu.tw/handle/123456789/464819
Title: | In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin | Authors: | Wu P.-C. Chen Y.-S. Hsieh T.-Y. Liu H.-W. Win-Li Lin Liu T.-M. |
Keywords: | Cell tracking; harmonic generation microscopy; melanoma; tumor microenvironments; two-photon fluorescence | Issue Date: | 2012 | Journal Volume: | 8233 | Source: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Abstract: | The understanding of the interaction between tumors and surrounding microenvironment in vivo is an important first step and basis for pathway-targeting cancer therapy. To in vivo observe the dynamic development of tumor cells and validate the efficacy of therapy in microscopic scales, people commonly performed multi-photon fluorescence microscopy through an invasive window chamber setup. However, under such system, the cancer cells can't be identified and long-term tracked without a fluorescence labeling. Exploiting the intrinsic third harmonic generation (THG) and two-photon fluorescence (2PF) contrasts of melanin, we demonstrated in vivo identification of melanoma and tracked its development without labeling. It was achieved with a least invasive femtosecond Cr:forsterite laser and a laser scanning nonlinear microscopy system with 3D sub-micron spatial resolution. Combined with molecular probes or reporters, we anticipate thus developed platform a powerful tool to reveal molecular insights of tumor microenvironments, enhance our understanding of tumor biology, and trigger new therapeutic approaches. ? 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/464819 | ISSN: | 1605-7422 | DOI: | 10.1117/12.906682 | SDG/Keyword: | Cell tracking; harmonic generation microscopy; melanoma; Microenvironments; two-photon fluorescence; Dermatology; Fluorescence; Fluorescence microscopy; Harmonic generation; Medical applications; Nanoparticles; Photons; Probes; Tumors; Oncology |
Appears in Collections: | 醫學工程學研究所 |
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