https://scholars.lib.ntu.edu.tw/handle/123456789/464975
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wang N.-K. | en_US |
dc.contributor.author | Lai C.-C. | en_US |
dc.contributor.author | Liu C.-H. | en_US |
dc.contributor.author | Yeh L.-K. | en_US |
dc.contributor.author | Chou C.L. | en_US |
dc.contributor.author | Kong J. | en_US |
dc.contributor.author | Nagasaki T. | en_US |
dc.contributor.author | Tsang S.H. | en_US |
dc.contributor.author | CHUNG-LIANG CHIEN | en_US |
dc.creator | Wang N.-K.;Lai C.-C.;Liu C.-H.;Yeh L.-K.;Chou C.L.;Kong J.;Nagasaki T.;Tsang S.H.;Chung-Liang Chien | - |
dc.date.accessioned | 2020-02-27T01:50:07Z | - |
dc.date.available | 2020-02-27T01:50:07Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1754-8403 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883886303&doi=10.1242%2fdmm.012112&partnerID=40&md5=4a5b5c06ac3ada68a819027b19d17113 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/464975 | - |
dc.description.abstract | Goldmann-Favre syndrome, also known as enhanced S-cone syndrome, is an inherited retinal degeneration disease in which a gain of photoreceptor cell types results in retinal dysplasia and degeneration. Although microglia have been implicated in the pathogenesis of many neurodegenerative diseases, the fundamental role of these cells in this disease is unknown. In the current study, sequential analyses suggest that microglia are recruited and appear after outer nuclear layer folding. By crossing rd7 mice (a model for hereditary retinal degeneration owing to Nr2e3 mutation) with mice carrying the macrophage Fas-induced apoptosis (Mafia) transgene, we generated double-mutant mice and studied the role of the resident retinal microglia. Microglial cells in these double-mutant mice express enhanced green fluorescent protein (EGFP) and a suicide gene that can trigger Fasmediated apoptosis via systemic treatment with AP20187 (FK506 dimerizer). We demonstrated that more than 80% of the EGFP+ cells in retinas from rd7/rd7;Tg/Tg mice express Iba-1 (a microglial marker), and resident microglia are still present in the retina because AP20187 does not cross the blood-brain barrier. Hence, only circulating bone marrow (BM)-derived microglia are depleted. Depletion of circulating BM-derived microglia accelerates retinal degeneration in rd7 mice. An increased number of autofluorescent (AF) spots is a consequence of resident microglia proliferation, which in turn establishes an inflammatory cytokine milieu via the upregulation of IL-1β, IL-6 and TNFα expression. This inflammation is likely to accelerate retinal degeneration. This study not only identifies inflammation as a crucial step in the pathogenesis of retinal degeneration, but also highlights the involvement of specific cytokine genes that could serve as future treatment targets in retinal degenerations. | - |
dc.relation.ispartof | DMM Disease Models and Mechanisms | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Fas ligand; interleukin 1beta; interleukin 6; tacrolimus; tumor necrosis factor alpha; animal experiment; animal model; apoptosis; article; autofluorescence; blood brain barrier; bone marrow cell; cell proliferation; controlled study; goldmann favre syndrome; macrophage; microglia; mouse; nonhuman; priority journal; retina degeneration; sequential analysis; suicide transgene; upregulation; Animals; Bone Marrow Cells; Cell Count; Cell Proliferation; Disease Models, Animal; Eye Diseases, Hereditary; Fluorescein Angiography; Gene Expression Regulation; Green Fluorescent Proteins; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microglia; Retinal Degeneration; Rod Cell Outer Segment; Tacrolimus; Time Factors; Vision Disorders | - |
dc.title | Origin of fundus hyperautofluorescent spots and their role in retinal degeneration in a mouse model of Goldmann-Favre syndrome | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1242/dmm.012112 | - |
dc.identifier.pmid | 23828046 | - |
dc.identifier.scopus | 2-s2.0-84883886303 | - |
dc.relation.pages | 1113-1122 | - |
dc.relation.journalvolume | 6 | - |
dc.relation.journalissue | 5 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Anatomy and Cell Biology | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.orcid | 0000-0001-9806-486X | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 解剖學暨細胞生物學科所 |
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