https://scholars.lib.ntu.edu.tw/handle/123456789/465377
標題: | Pearl extract enhances the migratory ability of fibroblasts in a wound healing model | 作者: | Li Y.-C Chen C.-R Tai-Horng Young |
關鍵字: | Migration; Pearl extraction (PL) | 公開日期: | 2013 | 卷: | 51 | 期: | 3 | 起(迄)頁: | 289-297 | 來源出版物: | Pharmaceutical Biology | 摘要: | Context: For 2000 years, traditional Chinese medicine has been used as a remedy for general health improvement, including the fight against aging. Pearl powder has recently been used as a health food that has antioxidant, antiaging, antiradioactive, and tonic activities for cells; it is also applied to cure aphthous ulcer, gastric ulcer, and duodenal ulcer on clinical therapy. In addition, the mother of pearl, nacre, could enhance the cell adhesion and tissue regeneration of skin fibroblasts. Objective: Fibroblast is regarded as indispensable in the processes of wound healing. Therefore, the effect of pearl extract (PL) on fibroblasts is investigated in this study. Materials and methods: PL is produced by a room temperature super extraction system (Taiwan patent no. I271 220). DMEM medium containing PL (300 μg/mL) was used to examine the effect of migration-promoting potential on human fibroblast cell line or human primary fibroblast cells in a wound healing model in vitro. Results: Medium containing PL (300 μg/mL) demonstrated that the migratory cell numbers of fibroblasts were three times more than that without PL, and mRNA expression of collagen type III was higher than in collagen type I in fibroblasts. It revealed a migration-promoting potential of human fibroblasts in a wound healing model in vitro. Discussion and conclusion: The present study found that the migration-promoting effect in PL, which could be a supplement in cell culture. These data suggest PL could be useful for enhancing the wound healing of fibroblasts. ? 2013 Informa Healthcare USA, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465377 | ISSN: | 1388-0209 | DOI: | 10.3109/13880209.2012.721130 | SDG/關鍵字: | collagen type 1; collagen type 3; messenger RNA; natural product; pearl extract; unclassified drug; article; cell activity; cell count; cell migration; collagen type I gene; collagen type III gene; concentration response; controlled study; drug effect; drug mechanism; drug research; fibroblast culture; gene; gene expression; human; human cell; in vitro study; primary cell culture; room temperature; wound healing; Animals; Cell Line; Cell Movement; Cells, Cultured; Collagen Type III; Dermatologic Agents; Foreskin; Humans; Male; Materia Medica; Medicine, Chinese Traditional; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Skin; Unionidae; Up-Regulation; Wound Healing |
顯示於: | 醫學工程學研究所 |
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