https://scholars.lib.ntu.edu.tw/handle/123456789/465466
標題: | A surgical model of fulminant hepatic failure in rabbits | 作者: | Hung K.-C Yong C.-C Chen Y.-S Eng H.-L Kuo F.-Y Lin C.-C Tai-Horng Young Kobayashi E Chen C.-L Wang C.-C. |
關鍵字: | Animal model; Hepatic encephalopathy; Intracranial pressure | 公開日期: | 2007 | 卷: | 27 | 期: | 10 | 起(迄)頁: | 1333-1341 | 來源出版物: | Liver International | 摘要: | Aim: Animal models of fulminant hepatic failure (FHF) have been developed for characterization of disease progression and to evaluate the effectiveness of liver-assist devices, some by treatment with hepatotoxic drugs, viral hepatitis or surgical procedures. We have developed a model in the rabbit by combining resection of the three anterior lobes with ligation of the pedicle of the right lateral lobes, resulting in liver necrosis; the remnant quadrate lobes are left intact. Materials and methods: Adult male New Zealand white rabbits (n = 16) were used. Six animals were killed to measure the weight of the separate liver lobes. The others (n = 10) underwent left neck central line placement to monitor continuous blood pressure and collect blood for laboratory analysis, and a burr hole on the right parietal bone to monitor the intracranial pressure (ICP). Blood laboratory analysis, clinical hepatic encephalopathy and ICP levels were measured in FHF animals (n = 6). Animals (n = 4) undergoing a sham operation served as controls. Results: All FHF animals died between 12 and 26 h after liver surgery from FHF characterized by a progressive increase in liver enzymes, ammonia, total bilirubin, coagulopathy, hepatic encephalopathy and intracranial hypertension. Histological features of the ischaemic lobes showed coagulative necrosis of hepatocytes with absence of nuclei and collapse of cell plates. Brain histology revealed hypoxic cell damage. Conclusion: We have developed a simple, reproducible model of FHF in rabbits that has a number of features comparable with clinical FHF patients and is well suited for testing experimental bioartificial liver systems and investigating the pathogenesis of FHF. ? 2007 Blackwell Munksgaard. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465466 | ISSN: | 1478-3223 | DOI: | 10.1111/j.1478-3231.2007.01512.x | SDG/關鍵字: | ammonia; bilirubin; liver enzyme; animal experiment; animal model; animal tissue; article; artificial liver; blood clotting disorder; blood pressure monitoring; cell damage; cell hypoxia; cell nucleus; controlled study; hepatic encephalopathy; histopathology; intracranial hypertension; intracranial pressure; laboratory test; ligation; liver cell; liver failure; liver ischemia; liver necrosis; liver weight; male; nonhuman; pathogenesis; rabbit; reproducibility; Animals; Disease Models, Animal; Disease Progression; Hepatectomy; Hepatic Encephalopathy; Intracranial Pressure; Liver Failure, Acute; Male; Necrosis; Organ Size; Rabbits |
顯示於: | 醫學工程學研究所 |
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