|Title:||BSA-bounded p-cresyl sulfate potentiates the malignancy of bladder carcinoma by triggering cell migration and EMT through the ROS/Src/FAK signaling pathway||Authors:||Peng Y.-S.
|Issue Date:||2019||Publisher:||Springer||Source:||Cell Biology and Toxicology||Abstract:||
Para-cresyl sulfate (P-CS), a major uremic toxin derived from the metabolites of tyrosine and phenylalanine through liver, existed in the blood of patients with chronic kidney disease (CKD). CKD increases the malignancy in bladder cancers; however, effects of P-CS on bladder cancers are not fully understood. P-CS is conjugated with BSA physiologically, and this study aims to investigate the effects and possible underlying mechanisms of BSA-bounded P-CS on human bladder cancer cells. With P-CS treatment, the intracellular ROS increased in bladder cancer cells. ROS then triggered epithelial-mesenchymal transition (EMT), stress fiber redistribution, and cell migration. With specific inhibitors, the key signals regulating P-CS-treated migration are Src and FAK. This study provided a clinical clue that patients with higher serum P-CS have a higher risk of malignant urothelial carcinomas, and a regulatory pathway of how P-CS regulates bladder cancer migration. ? 2019, Springer Nature B.V.
|ISSN:||0742-2091||DOI:||10.1007/s10565-019-09509-0||metadata.dc.subject.other:||focal adhesion kinase; para cresyl sulfate; protein kinase p60; reactive oxygen metabolite; unclassified drug; uremic toxin; protein tyrosine kinase; reactive oxygen metabolite; sulfate; actin filament; Article; bladder carcinoma; cell migration; cell survival; controlled study; epithelial mesenchymal transition; flow cytometry; human; human cell; immunofluorescence; phase contrast microscopy; priority journal; protein expression; signal transduction; bladder; bladder tumor; cell motion; drug effect; epithelial mesenchymal transition; epithelium cell; metabolism; pathology; physiology; tumor cell line; Cell Line, Tumor; Cell Movement; Epithelial Cells; Epithelial-Mesenchymal Transition; Humans; Reactive Oxygen Species; Signal Transduction; src-Family Kinases; Sulfates; Urinary Bladder; Urinary Bladder Neoplasms
|Appears in Collections:||解剖學暨細胞生物學科所|
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