https://scholars.lib.ntu.edu.tw/handle/123456789/465715
標題: | PH-Responsive Nanophotosensitizer for an Enhanced Photodynamic Therapy of Colorectal Cancer Overexpressing EGFR | 作者: | Chu W.-Y. Tsai M.-H. Peng C.-L. Shih Y.-H. Luo T.-Y. Yang S.-J. MING-JIUM SHIEH |
關鍵字: | Ce6; epidermal growth factor receptor; micelle; Photodynamic therapy | 公開日期: | 2018 | 出版社: | American Chemical Society | 卷: | 15 | 期: | 4 | 起(迄)頁: | 1432-1444 | 來源出版物: | Molecular Pharmaceutics | 摘要: | Photodynamic therapy (PDT) has been shown to kill cancer cells and improve survival and quality of life in cancer patients, and numerous new approaches have been considered for maximizing the efficacy of PDT. In this study, a new multifunctional nanophotosensitizer Ce6/GE11- (pH) micelle was developed to target epidermal growth factor receptor (EGFR) overexpressing colorectal cancer (CRC) cells. This nanophotosensitizer was synthesized using a micelle comprising pH-responsive copolymers (PEGMA-PDPA), biodegradable copolymers (mPEG-PCL), and maleimide-modified biodegradable copolymers (Mal-PEG-PCL) to entrap the potential hydrophobic photosensitizer chlorin e6 (Ce6) and to present EGFR-targeting peptides (GE11) on its surface. In the presence of Ce6/GE11- (pH) micelles, Ce6 uptake by EGFR-overexpressing CRC cells significantly increased due to GE11 specificity. Moreover, Ce6 was released from Ce6/GE11- (pH) micelles in tumor environments, leading to improved elimination of cancer cells in PDT. These results indicate enhanced efficacy of PDT using Ce6/GE11- (pH) micelle, which is a powerful nanophotosensitizer with high potential for application to future PDT for CRC. ? 2018 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044717281&doi=10.1021%2facs.molpharmaceut.7b00925&partnerID=40&md5=fecfc8cdc564efee4dc3328579fd8b0d https://scholars.lib.ntu.edu.tw/handle/123456789/465715 |
ISSN: | 1543-8384 | DOI: | 10.1021/acs.molpharmaceut.7b00925 | SDG/關鍵字: | copolymer; epidermal growth factor receptor; maleimide; photosensitizing agent; singlet oxygen; epidermal growth factor receptor; nanoparticle; peptide; photosensitizing agent; polymer; absorption spectroscopy; animal experiment; animal model; animal tissue; Article; cell proliferation; cell viability; chemical structure; colorectal cancer; controlled study; critical micelle concentration; cytotoxicity; drug synthesis; electron spin resonance; ex vivo study; female; fluorescence spectroscopy; immunohistochemistry; in vitro study; in vivo study; incubation time; miscibility; molecular weight; mouse; MTT assay; nonhuman; pH; photocytotoxicity; photodynamic therapy; priority journal; proton nuclear magnetic resonance; proton transport; surface property; transmission electron microscopy; tumor microenvironment; tumor volume; animal; chemistry; colorectal tumor; HCT 116 cell line; human; metabolism; micelle; pH; tumor cell line; Animals; Cell Line, Tumor; Colorectal Neoplasms; ErbB Receptors; HCT116 Cells; Humans; Hydrogen-Ion Concentration; Mice; Micelles; Nanoparticles; Peptides; Photosensitizing Agents; Polymers |
顯示於: | 醫學工程學研究所 |
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