https://scholars.lib.ntu.edu.tw/handle/123456789/465782
標題: | Development of pH sensitive 2-(diisopropylamino)ethyl methacrylate based nanoparticles for photodynamic therapy | 作者: | Peng C.-L. Yang L.-Y. Luo T.-Y. Lai P.-S. Yang S.-J. Lin W.-J. MING-JIUM SHIEH |
公開日期: | 2010 | 卷: | 21 | 期: | 15 | 來源出版物: | Nanotechnology | 摘要: | Photodynamic therapy is an effective treatment for tumors that involves the administration of light-activated photosensitizers. However, most photosensitizers are insoluble and non-specific. To target the acid environment of tumor sites, we synthesized three poly(ethylene glycol) methacrylate-co-2- (diisopropylamino)ethyl methacrylate (PEGMA-co-DPA) copolymers capable of self-assembly to form pH sensitive nanoparticles in an aqueous environment, as a means of encapsulating the water-insoluble photosensitizer, meso-tetra(hydroxyphenyl)chlorin (m-THPC). The critical aggregation pH of the PEGMA-co-DPA polymers was 5.8-6.6 and the critical aggregation concentration was 0.0045-0.0089wt% at pH 7.4. Using solvent evaporation, m-THPC loaded nanoparticles were prepared with a high drug encapsulation efficiency (approximately 89%). Dynamic light scattering and transmission electron microscopy revealed the spherical shape and 132nm diameter of the nanoparticles. The invitro release rate of m-THPC at pH 5.0 was faster than at pH 7.0 (58% versus 10% m-THPC released within 48h, respectively). The invitro photodynamic therapy efficiency was tested with the HT-29 cell line. m-THPC loaded PEGMA-co-DPA nanoparticles exhibited obvious phototoxicity in HT-29 colon cancer cells after light irradiation. The results indicate that these pH sensitive nanoparticles are potential carriers for tumor targeting and photodynamic therapy. ? 2010 IOP Publishing Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949884106&doi=10.1088%2f0957-4484%2f21%2f15%2f155103&partnerID=40&md5=5a0bd83f48bfd9bf51f7413cfd5e14c4 https://scholars.lib.ntu.edu.tw/handle/123456789/465782 |
ISSN: | 0957-4484 | DOI: | 10.1088/0957-4484/21/15/155103 | SDG/關鍵字: | Acid environment; Aqueous environment; Cell lines; Chlorins; Colon cancer cells; Critical aggregation; Critical aggregation concentration; Drug encapsulation efficiency; Ethyl methacrylates; In-vitro; Light irradiations; pH sensitive; Photo-toxicity; Poly(ethylene glycol) methacrylate; Release rate; Solvent evaporation; Spherical shape; Tumor site; Tumor targeting; Cell culture; Complexation; Encapsulation; Ethylene; Ethylene glycol; Light transmission; Nanoparticles; Optical materials; pH sensors; Photosensitizers; Plasmons; Polyethylene glycols; Polyethylene oxides; Scanning electron microscopy; Transmission electron microscopy; Tumors; Photodynamic therapy; macrogol derivative; mesoporphyrin; methacrylic acid derivative; nanoparticle; poly(2 (diisopropylamino)ethyl methacrylate); poly(2-(diisopropylamino)ethyl methacrylate); polyethylene glycol methacrylate; polymethacrylic acid derivative; temoporfin; animal; article; cell death; cell fractionation; cell strain 3T3; cell strain HT29; cell survival; chemistry; drug effect; human; intracellular space; light; metabolism; mouse; nuclear magnetic resonance spectroscopy; particle size; pH; photochemotherapy; radiation exposure; spectrofluorometry; synthesis; transport at the cellular level; ultrastructure; Animals; Biological Transport; Cell Death; Cell Survival; HT29 Cells; Humans; Hydrogen-Ion Concentration; Intracellular Space; Light; Magnetic Resonance Spectroscopy; Mesoporphyrins; Methacrylates; Mice; Nanoparticles; NIH 3T3 Cells; Particle Size; Photochemotherapy; Polyethylene Glycols; Polymethacrylic Acids; Spectrometry, Fluorescence; Subcellular Fractions |
顯示於: | 醫學工程學研究所 |
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