https://scholars.lib.ntu.edu.tw/handle/123456789/465922
Title: | SDF-1/CXCR4 Signaling Maintains Stemness Signature in Mouse Neural Stem/Progenitor Cells | Authors: | Ho, Shih-Yin THAI-YEN LING Lin, Hsing-Yu Liou, Jeffrey Tsai-Jui Liu, Fei-Chih Chen, I-Chun Lee, Sue-Wei Hsu, Yu DAR-MING LAI HORNG-HUEI LIOU |
Issue Date: | 2017 | Journal Volume: | 2017 | Start page/Pages: | 2493752 | Source: | Stem Cells International | Abstract: | SDF-1 and its primary receptor, CXCR4, are highly expressed in the embryonic central nervous system (CNS) and play a crucial role in brain architecture. Loss of SDF-1/CXCR4 signaling causes abnormal development of neural stem/progenitor cells (NSCs/NPCs) in the cerebellum, hippocampus, and cortex. However, the mechanism of SDF-1/CXCR4 axis in NSCs/NPCs regulation remains unknown. In this study, we found that elimination of SDF-1/CXCR4 transduction caused NSCs/NPCs to lose their stemness characteristics and to encounter neurogenic differentiation. Moreover, Notch and RE1 silencing transcription factor (REST) both play an essential role in NSCs/NPCs maintenance and neuronal differentiation and were dramatically downregulated following SDF-1/CXCR4 cascade inhibition. Finally, we demonstrated that the expression of achaete-scute homolog 1 (Ascl1), a proneural gene, and p27, an antiproliferative gene, were significantly increased after genetic elimination of SDF-1 alleles. Our results support that the loss of functional SDF-1/CXCR4 signaling pathway in NSCs/NPCs induces exit of cell cycle and promotes premature neural differentiation. © 2017 Shih-Yin Ho et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/465922 | ISSN: | 16879678 | DOI: | 10.1155/2017/2493752 |
Appears in Collections: | 藥理學科所 |
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