https://scholars.lib.ntu.edu.tw/handle/123456789/467583
標題: | Bioanalytical system for detection of cancer cells with photoluminescent ZnO nanorods | 作者: | Viter, R. Jekabsons, K. Kalnina, Z. Poletaev, N. Hsu, S.H. Riekstina, U. SHAN-HUI HSU |
關鍵字: | cancer detection; carbohydrate marker; photoluminescence; SSEA-4; ZnO nanorods | 公開日期: | 2016 | 卷: | 27 | 期: | 46 | 來源出版物: | Nanotechnology | 摘要: | Using photoluminescent ZnO nanorods and carbohydrate marker SSEA-4, a novel cancer cell recognition system was developed. Immobilization of SSEA-4 antibodies (αSSEA-4) on ZnO nanorods was performed in buffer solution (pH = 7.1) over 2 h. The cancer cell line probes were fixed on the glass slide. One hundred microliters of ZnO-αSSEA-4 conjugates were deposited on the cell probe and exposed for 30 min. After washing photoluminescence spectra were recorded. Based on the developed methodology, ZnO-αSSEA-4 probes were tested on patient-derived breast and colorectal carcinoma cells. Our data clearly show that the carbohydrate SSEA-4 molecule is expressed on cancer cell lines and patient-derived cancer cells. Moreover, SSEA-4 targeted ZnO nanorods bind to the patient-derived cancer cells with high selectivity and the photoluminescence signal increased tremendously compared to the signal from the control samples. Furthermore, the photoluminescence intensity increase correlated with the extent of malignancy in the target cell population. A novel portable bioanalytical system, based on optical ZnO nanorods and fiber optic detection system was developed. We propose that carbohydrate SSEA-4 specific ZnO nanorods could be used for the development of cancer diagnostic biosensors and for targeted therapy. ? 2016 IOP Publishing Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/467583 | DOI: | 10.1088/0957-4484/27/46/465101 | SDG/關鍵字: | Carbohydrates; Cell culture; Cell immobilization; Cell proliferation; Diseases; II-VI semiconductors; Nanorods; Photoluminescence; Probes; Zinc oxide; Bioanalytical systems; Cancer detection; Colorectal carcinoma; Photoluminescence intensities; Photoluminescence signals; Photoluminescence spectrum; SSEA-4; ZnO nanorod; Cells; antibody; nanotube; zinc oxide; genetic procedures; human; luminescence; neoplasm; Antibodies; Biosensing Techniques; Humans; Luminescence; Nanotubes; Neoplasms; Zinc Oxide |
顯示於: | 高分子科學與工程學研究所 |
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