https://scholars.lib.ntu.edu.tw/handle/123456789/468496
標題: | Adipose-Derived Stem Cells Protect Skin Flaps against Ischemia/Reperfusion Injury via IL-6 Expression | 作者: | Pu C.-M. Liu C.-W. Liang C.-J. Yen Y.-H. Chen S.-H. Jiang-Shieh Y.-F. CHUNG-LIANG CHIEN Chen Y.-C. YUH-LIEN CHEN |
公開日期: | 2017 | 出版社: | Elsevier B.V. | 卷: | 137 | 期: | 6 | 起(迄)頁: | 1353-1362 | 來源出版物: | Journal of Investigative Dermatology | 摘要: | Flap necrosis is the most frequent postoperative complication encountered in reconstructive surgery. We elucidated whether adipose-derived stem cells (ADSCs) and their derivatives might induce neovascularization and protect skin flaps during ischemia/reperfusion (I/R) injury. Flaps were subjected to 3 hours of ischemia by ligating long thoracic vessels and then to blood reperfusion. Qtracker-labeled ADSCs, ADSCs in conditioned medium (ADSC-CM), or ADSC exosomes (ADSC-Exo) were injected into the flaps. These treatments led to significantly increased flap survival and capillary density compared with I/R on postoperative day 5. IL-6 levels in the cell lysates or in conditioned medium were significantly higher in ADSCs than in Hs68 fibroblasts. ADSC-CM and ADSC-Exo increased tube formation. This result was corroborated by a strong decrease in skin repair after adding IL-6–neutralizing antibodies or small interfering RNA for IL-6 ADSCs. ADSC transplantation also increased flap recovery in I/R injury of IL-6–knockout mice. IL-6 was secreted from ADSCs through signal transducer and activator of transcription phosphorylation, and then IL-6 stimulated angiogenesis and enhanced recovery after I/R injury by the classic signaling pathway. The mechanism of skin recovery includes the direct differentiation of ADSCs into endothelial cells and the indirect effect of IL-6 released from ADSCs. ADSC-CM and ADSC-Exo could be used as off-the-shelf products for this therapy. ? 2017 The Authors |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019897244&doi=10.1016%2fj.jid.2016.12.030&partnerID=40&md5=62a846cc512e61691b5a99042d98096b https://scholars.lib.ntu.edu.tw/handle/123456789/468496 |
ISSN: | 0022-202X | DOI: | 10.1016/j.jid.2016.12.030 | SDG/關鍵字: | interleukin 6; mitogen activated protein kinase; small interfering RNA; STAT3 protein; interleukin 6; STAT3 protein; STAT3 protein, human; adipose derived stem cell; adult; angiogenesis; animal experiment; Article; capillary density; cell differentiation; cell lysate; conditioned medium; controlled study; cytokine release; endothelium cell; human; male; mouse; neovascularization (pathology); nonhuman; postoperative period; priority journal; protein expression; protein phosphorylation; reperfusion injury; skin fibroblast; skin flap; skin flap survival; stem cell transplantation; upregulation; adipose tissue; angiogenesis; animal; C57BL mouse; cell culture; cytology; disease model; fibroblast; genetics; graft survival; immunohistochemistry; immunology; metabolism; pathology; phosphorylation; procedures; randomization; reperfusion injury; stem cell; stem cell transplantation; surgical flaps; Adipose Tissue; Animals; Cells, Cultured; Disease Models, Animal; Fibroblasts; Graft Survival; Immunohistochemistry; Interleukin-6; Male; Mice; Mice, Inbred C57BL; Neovascularization, Physiologic; Phosphorylation; Random Allocation; Reperfusion Injury; STAT3 Transcription Factor; Stem Cell Transplantation; Stem Cells; Surgical Flaps |
顯示於: | 解剖學暨細胞生物學科所 |
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