https://scholars.lib.ntu.edu.tw/handle/123456789/468505
標題: | Ganoderma lucidum polysaccharides reduce lipopolysaccharide-induced interleukin-1 β expression in cultured smooth muscle cells and in thoracic aortas in mice | 作者: | Liang C.-J. Lee C.-W. Sung H.-C. Chen Y.-H. Chiang Y.-C. Hsu H.-Y. Tseng Y.-C. Li C.-Y. SHU-HUEI WANG YUH-LIEN CHEN |
公開日期: | 2014 | 出版社: | Oxford University Press | 卷: | 2014 | 起(迄)頁: | 305149 | 來源出版物: | Evidence-based Complementary and Alternative Medicine | 摘要: | The expression of inflammatory cytokines on vascular walls is a critical event in vascular diseases and inflammation. The aim of the present study was to examine the effects of an extract of Ganoderma lucidum (Reishi) polysaccharides (EORPs), which is effective against immunological disorders, on interleukin- (IL-) 1β expression by human aortic smooth muscle cells (HASMCs) and the underlying mechanism. The lipopolysaccharide- (LPS-) induced IL-1β expression was significantly reduced when HASMCs were pretreated with EORP by Western blot and immunofluorescent staining. Pretreatment with 10 g/mL EORP decreased LPS-induced ERK, p38, JNK, and Akt phosphorylation. But the increase in IL-1β expression with LPS treatment was only inhibited by pretreatment with the ERK1/2 inhibitor, while the JNK and p38 inhibitors had no effect. In addition, EORP reduced the phosphorylation and nuclear translocation of nuclear factor- (NF-) κB p65 in LPS-treated HASMCs. Furthermore, in vivo, IL-1β expression was strongly expressed in thoracic aortas in LPS-treated mice. Oral administration of EORP decreased IL-1β expression. The level of IL-1β expression in LPS-treated or in LPS/EORP-treated group was very low and was similar to that of the saline-treated group in toll-like receptor 4-deficient (TLR4-/-) mice. These findings suggest that EORP has the anti-inflammatory property and could prove useful in the prevention of vascular diseases and inflammatory responses. ? 2014 Chan-Jung Liang et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896479911&doi=10.1155%2f2014%2f305149&partnerID=40&md5=a69467f5ea1b3436f6c213a8465c782c https://scholars.lib.ntu.edu.tw/handle/123456789/468505 |
ISSN: | 1741-427X | DOI: | 10.1155/2014/305149 | SDG/關鍵字: | antiinflammatory agent; Ganoderma lucidum extract; interleukin 1beta; lipopolysaccharide; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; polysaccharide; sodium chloride; toll like receptor 4; animal cell; animal experiment; animal model; antiinflammatory activity; article; cardiovascular disease; cell culture; cell viability; controlled study; Ganoderma lucidum; immunofluorescence; in vitro study; in vivo study; inflammatory disease; mouse; nonhuman; priority journal; protein expression; protein phosphorylation; smooth muscle fiber; thoracic aorta; Western blotting |
顯示於: | 解剖學暨細胞生物學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。