https://scholars.lib.ntu.edu.tw/handle/123456789/469802
標題: | OxLDL upregulates caveolin-1 expression in macrophages: Role for caveolin-1 in the adhesion of oxLDL-treated macrophages to endothelium | 作者: | CHAU-CHUNG WU SHU-HUEI WANG Kuan I.-I. Tseng, Wei-Kung MING-FONG CHEN Wu, Jiahn-Chun YUH-LIEN CHEN |
關鍵字: | Caveolin-1; Hypercholesterolemia; Macrophages; MAPKs; oxLDL | 公開日期: | 1-六月-2009 | 卷: | 107 | 期: | 3 | 起(迄)頁: | 460-472 | 來源出版物: | Journal of Cellular Biochemistry | 摘要: | Caveolin-1, a principle component of caveolae, is present in several cell types known to play an important role in the development of atherosclerosis. In this study, its distribution and expression were studied in the arterial walls of hypercholesterolemic rabbits and apo-E-deficient mice and in oxidized low-density lipoprotein (oxLDL)-treated RAW264.7 macrophages. Immunohistochemical studies showed that staining for caveolin-1 expression was stronger in atherosclerotic lesions in hypercholesterolemic rabbits and apo-E-deficient mice compared to normal rabbits and mice and was closely associated with macrophages. OxLDL treatment increased caveolin-1 protein expression in RAW264.7 macrophages in a time- and dose-dependent manner. The increase in caveolin-1 expression was dependent on phosphorylation of the mitogen-activated protein kinases (MAPKs) extracellular signal-regulated kinase1/2 (ERK1/2), p38, and Jun N-terminal kinase (JNK) and the transcriptional activation and translocation of nuclear factor-κB (NF-κB). OxLDL also induced caveolin-1 mRNA expression and this effect was not seen in the presence of inhibitors for transcription or de novo protein synthesis. OxLDL increased the adhesion of RAW264.7 macrophages to endothelial cells via an increase in caveolin-1 expression, and the adhesion was reduced by the use of anti-caveolin-1 antibody or caveolin-1-specific shRNA. These results show that oxLDL increases caveolin-1 expression in macrophages through the MAPKs/NF-κB pathway. The caveolin-1 levels are closely associated with the adherence of monocytes/macrophages to endothelial cells and their accumulation within the arterial intima after hypercholesterolemia insult, resulting in the progression of atherosclerosis. © 2009 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-66749135266&doi=10.1002%2fjcb.22144&partnerID=40&md5=4c9ff31d0f2a074fbbdf1babfa4b648d https://scholars.lib.ntu.edu.tw/handle/123456789/469802 |
ISSN: | 0730-2312 | DOI: | 10.1002/jcb.22144 |
顯示於: | 醫學教育暨生醫倫理學科所 |
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