https://scholars.lib.ntu.edu.tw/handle/123456789/473572
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHING-HUNG LIN | en_US |
dc.contributor.author | Liu J.M. | en_US |
dc.contributor.author | YEN-SHEN LU | en_US |
dc.contributor.author | Lan C. | en_US |
dc.contributor.author | Lee W.-C. | en_US |
dc.contributor.author | KUAN-TING KUO | en_US |
dc.contributor.author | Wang C.-C. | en_US |
dc.contributor.author | DWANG-YING CHANG | en_US |
dc.contributor.author | CHIUN-SHENG HUANG | en_US |
dc.contributor.author | ANN-LII CHENG | en_US |
dc.creator | Cheng A.L.;Huang C.-S.;Chang D.-Y.;Wang C.-C.;KUAN-TING KUO;Lee W.-C.;Lan C.;Lu Y.-S.;Liu J.M.;Lin C.-H. | - |
dc.date.accessioned | 2020-03-07T06:42:13Z | - |
dc.date.available | 2020-03-07T06:42:13Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0021-9746 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874656965&doi=10.1136%2fjclinpath-2012-200929&partnerID=40&md5=4d5692fedfeeec0f9c32a976ca596f6f | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/473572 | - |
dc.description.abstract | Aims: The ESR1 gene encodes for oestrogen receptor (ER) α, which plays a crucial role in mammary carcinogenesis and clinical outcome in patients with breast cancer. However, the clinical significance of the ESR1 gene copy number change for breast cancer has not been clarified. Methods: ESR1 gene copy number was determined by fluorescence in situ hybridisation (FISH) on tissue sections. A minimum of 20 tumour cells were counted per section, and a FISH ratio of ESR1 gene to CEP6 ?2.0 was considered ESR1 amplification. A ratio >1.2 but <2.0 was considered ESR1 gain. The ESR1 copy number was further measured by quantitative real-time PCR (Q-PCR) with ASXL2 as a reference. Results: FISH revealed ESR1 amplification in six cases (4.0%) and ESR1 gain in 13 cases (8.7%) from a total of 150 cases. ESR1 gain and amplification were more common in older patients (p<0.001), and correlated well with ER protein expression (p=0.03) measured by immunohistochemistry, and ESR1 copy number (p<0.001) measured by Q-PCR. Furthermore, the multivariate analysis revealed that ESR1 amplification was associated with a shorter disease-free survival (HR=5.56, p=0.03) and a shorter overall survival (HR=5.11, p=0.04). Conclusions: In general, the frequency of ESR1 amplification in breast cancer is low when measured by FISH in large sections. ESR1 gain and amplification in breast cancer may be associated with older age and poorer outcomes. | - |
dc.relation.ispartof | Journal of Clinical Pathology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | estrogen receptor alpha; adult; article; breast cancer; breast carcinogenesis; cancer grading; cancer survival; clinical trial; congenital erythropoietic porphyria; controlled study; disease free survival; female; fluorescence in situ hybridization; gene amplification; gene dosage; genetic gain; human; human cell; human tissue; immunohistochemistry; major clinical study; overall survival; priority journal; protein expression; real time polymerase chain reaction; tissue section; tumor cell; Adult; Age Factors; Breast Neoplasms; Chi-Square Distribution; Disease-Free Survival; DNA Copy Number Variations; Estrogen Receptor alpha; Female; Gene Amplification; Gene Dosage; Genetic Predisposition to Disease; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Kaplan-Meier Estimate; Middle Aged; Multivariate Analysis; Phenotype; Predictive Value of Tests; Prognosis; Proportional Hazards Models; Real-Time Polymerase Chain Reaction; Repressor Proteins; Time Factors; Tumor Markers, Biological | - |
dc.title | Clinical significance of ESR1 gene copy number changes in breast cancer as measured by fluorescence in situ hybridisation | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1136/jclinpath-2012-200929 | - |
dc.identifier.pmid | 23268322 | - |
dc.identifier.scopus | 2-s2.0-84874656965 | - |
dc.relation.pages | 140-145 | - |
dc.relation.journalvolume | 66 | - |
dc.relation.journalissue | 2 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Medical Oncology-NTUCC | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Clinical Trial Center | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Pathology | - |
crisitem.author.dept | Pathology-NTUH | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Oncology-NTUH | - |
crisitem.author.orcid | 0000-0003-2403-4056 | - |
crisitem.author.orcid | 0000-0001-7461-1291 | - |
crisitem.author.orcid | 0000-0002-3657-1652 | - |
crisitem.author.orcid | 0000-0002-6557-211X | - |
crisitem.author.orcid | 0000-0002-9152-6512 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 病理學科所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。