https://scholars.lib.ntu.edu.tw/handle/123456789/473603
標題: | Increased Ki-67 proliferative index and absence of P16INK4 in CIN-HPV related pathogenic pathways different from cervical squamous intraepithelial lesion | 作者: | KUAN-TING KUO Chang H.-C. Hsiao C.-H. Lin M.-C. |
公開日期: | 2006 | 卷: | 90 | 期: | 7 | 起(迄)頁: | 894-899 | 來源出版物: | British Journal of Ophthalmology | 摘要: | Background/aim: It is generally assumed that similar pathways are involved in human papillomavirus (HFV) induced pathogenesis of cervical squamous intraepithelial lesions (SILs) and cancers and a subset of conjunctival intraepithelial neoplasm (CIN)-that the malignancies or pre-cancerous lesions arise through HPV oncoproteins E6 and E7, which disrupt the pathways of p53 and the product of the retinoblastoma (Rb) gene and, in turn, increase the protein product of gene p16INK4 through the mechanism of positive feedback. Several cell cycle molecules are detected to test this hypothesis. Methods: Nine cases of CIN and eight non-CIN cases were analysed for the expression of Ki-67, pRb, p53, and p16INK4 via immunohistochemistry. Nine cases of cervical high grade squamous intraepithelial lesion (HSIL), and 10 cases of cervical low grade squamous intraepithelial lesion (LSIL) were included for stain control of p16INK4a, and comparison of p16INK4a expression to CIN cases. A nested polymerase chain reaction and a genechip HPV typing were used to detect HPV infection and types in the CIN and non-CIN samples Results: HPV positivity was demonstrated in all of the CIN lesions but in none of the non-CIN lesions. The Ki-67 proliferative index (Ki-67 PI) was statistically higher in the CIN group than the non-CIN group; however, there were no differences of expression of pRb and p53 between the two groups and no expression of p16INK4 in all cases. All nine cases of HSIL, and seven out of 10 cases of LSIL used for stain control were immunoreactive for p16 INK4a. There were statistically significant differences in overexpression of p16INK4a between the CINs and SILs Conclusions: The Ki-67 proliferative index may be a sensitive marker for CIN lesions and these results, with significant differences in overexpression of p16INK4a between CINs and SILs, may provide new evidence that HPV related mucosal dysplasia in different anatomical locations may lead to dissimilar molecular pathways. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745668643&doi=10.1136%2fbjo.2005.086314&partnerID=40&md5=56013c734be278949b5e99bfca05dc69 https://scholars.lib.ntu.edu.tw/handle/123456789/473603 |
ISSN: | 0007-1161 | DOI: | 10.1136/bjo.2005.086314 | SDG/關鍵字: | cell cycle protein; Ki 67 antigen; protein p16INK4a; protein p53; retinoblastoma protein; adolescent; adult; aged; article; cancer grading; clinical article; conjunctiva epithelium; conjunctiva tumor; controlled study; female; histopathology; human; human tissue; immunohistochemistry; male; pathogenesis; polymerase chain reaction; priority journal; protein expression; sensitivity analysis; squamous epithelium; uterine cervix carcinoma in situ; virus detection; virus infection; Wart virus; Adolescent; Adult; Aged; Carcinoma in Situ; Cell Proliferation; Cervical Intraepithelial Neoplasia; Chi-Square Distribution; Conjunctival Neoplasms; Cyclin-Dependent Kinase Inhibitor p16; DNA, Viral; Epithelial Cells; Female; Humans; Immunohistochemistry; Ki-67 Antigen; Male; Middle Aged; Papillomaviridae; Papillomavirus Infections; Polymerase Chain Reaction; Tumor Markers, Biological; Uterine Cervical Neoplasms |
顯示於: | 病理學科所 |
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