https://scholars.lib.ntu.edu.tw/handle/123456789/473653
標題: | Expression of Rsf-1, a chromatin-remodeling gene, in ovarian and breast carcinoma | 作者: | TSUI-LIEN MAO Hsu C.-Y. Yen M.J. Gilks B. Sheu J.J.-C. Gabrielson E. Vang R. Cope L. Kurman R.J. Wang T.-L. Shih I.-M. |
關鍵字: | Breast carcinoma; Ovarian serous carcinoma; Rsf-1 | 公開日期: | 2006 | 卷: | 37 | 期: | 9 | 起(迄)頁: | 1169-1175 | 來源出版物: | Human Pathology | 摘要: | Rsf-1 protein is a member of a chromatin-remodeling complex that plays an important role in regulating gene expression and cell proliferation. Our previous study showed that Rsf-1 was an amplified gene that participated in the development of ovarian serous carcinoma. To further elucidate the role of Rsf-1 in ovarian cancer, we studied Rsf-1 immunoreactivity in 294 ovarian tumors of various histologic types. Because the Rsf-1 amplicon overlaps an amplified region reported in breast cancer, we included 782 neoplastic and normal breast tissues for comparison. Immunohistochemistry was performed on tissue microarrays using a 4-tiered scoring system. Overexpression of Rsf-1 was defined as a nuclear immunointensity of 3+ to 4+ because of a strong correlation between 3+ and 4+ immunointensity and Rsf-1 gene amplification, based on our previous fluorescence in situ hybridization analysis. Rsf-1 overexpression was observed in 25% of high-grade ovarian serous carcinomas and in only rare cases (<7%) of low-grade ovarian serous, ovarian endometrioid, and invasive breast carcinomas but not in any ovarian serous borderline tumors, ovarian clear cell carcinomas, ovarian mucinous carcinomas, intraductal carcinomas of the breast, and normal ovaries and breast tissues. Thus, overexpression of Rsf-1 was significantly associated with high-grade ovarian serous carcinoma (P < .05), as compared with other types of ovarian tumors and breast carcinomas. Our results provide evidence that Rsf-1 expression is primarily confined to high-grade serous carcinoma, the most aggressive ovarian cancer. Because Rsf-1 overexpression occurs in only a small number of breast carcinomas, it is unlikely that Rsf-1 is a critical gene in the development of breast carcinoma. ? 2006 Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747835279&doi=10.1016%2fj.humpath.2006.04.008&partnerID=40&md5=7326e054ddf1e68268cfe96b9d630753 https://scholars.lib.ntu.edu.tw/handle/123456789/473653 |
ISSN: | 0046-8177 | DOI: | 10.1016/j.humpath.2006.04.008 | SDG/關鍵字: | amplicon; article; breast; breast carcinoma; chromatin; chromatin assembly and disassembly; comparative study; controlled study; correlation analysis; endometrioid carcinoma; gene function; gene overexpression; histology; human; human tissue; immunohistochemistry; immunoreactivity; intraductal carcinoma; ovary carcinoma; ovary tumor; protein expression; scoring system; tissue microarray; Blotting, Western; Breast Neoplasms; Carcinoma; Chromatin Assembly and Disassembly; Female; Gene Amplification; Humans; Immunohistochemistry; Nuclear Proteins; Ovarian Neoplasms; Trans-Activators; Tumor Markers, Biological |
顯示於: | 病理學科所 |
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