https://scholars.lib.ntu.edu.tw/handle/123456789/477522
標題: | Effects of dietary glutamine on antioxidant enzyme activity and immune response in burned mice | 作者: | Yeh S.-L. Shang H.-F. MING-TSAN LIN Yeh C.-L. Chen W.-J. |
公開日期: | 2003 | 出版社: | Elsevier Inc. | 卷: | 19 | 期: | 10 | 起(迄)頁: | 880-885 | 來源出版物: | Nutrition | 摘要: | OBJECTIVES: We investigated the effect of dietary glutamine (Gln) on specific antibody production and antioxidant enzyme activities in burned mice vaccinated with detoxified Pseudomonas exotoxin A linked with the outer membrane proteins I and F (PEIF). We also evaluated the survival rate of vaccinated and non-vaccinated burned mice infected with Pseudomonas aeruginosa. METHODS: There were three consecutive experiments. In experiment 1, 30 BALB/c mice were assigned to one of two groups. The control group was fed casein as the protein source; the Gln group received 4% Gln (w/w) to replace part of the casein. Mice were immunized twice with PEIF, and the production of specific antibodies against PEIF was measured every week. Eight weeks after immunization, all mice received a 30% body surface area burn injury. Mice were killed 24 h after the burn. The antioxidant enzyme activities and lipid peroxides in the tissues and specific antibody production were analyzed. In experiment 2, 12 mice were assigned to a control or a Gln group and fed with one the experimental diets for 4 wk. Then burn injury was induced, and mice were killed 24 h later. In vitro, splenocytes were cultured, and interleukin (IL)-4 and IL-10 were measured after mitogen stimulation. In experiment 3, survival rates of vaccinated and non-vaccinated burned mice complicated with P. aeruginosa infection were evaluated. The survival rate was observed for 8 d after the burn. RESULTS: Antioxidant enzyme activities and lipid peroxides in tissues tended to be lower in the Gln group than in the control group after the burn. Specific antibody production against P. aeruginosa increased significantly in the Gln group at 4 and 7 wk after immunization and at 24 h after the burn. IL-4 concentrations in mitogen-stimulated splenocytes were significantly higher in the Gln group than in the control group. Survival rates of non-vaccinated burned mice in the Gln group were significantly higher than those in the control group, whereas there was no difference in the survival of vaccinated burned mice after bacterial infection. CONCLUSIONS: These results suggested that vaccinated mice receiving a Gln-enriched diet may have enhanced humoral immunity and attenuated oxidative stress induced by burn injury. Also, Gln supplementation improved the survival of burned mice complicated with P. aeruginosa infection. ? Elsevier Inc. 2003. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0141987852&doi=10.1016%2fS0899-9007%2803%2900159-X&partnerID=40&md5=03c3f078ad23b9d7e12e6b993b27ddb1 https://scholars.lib.ntu.edu.tw/handle/123456789/477522 |
ISSN: | 0899-9007 | DOI: | 10.1016/S0899-9007(03)00159-X | SDG/關鍵字: | antioxidant; casein; glutamine; glutathione peroxidase; interleukin 10; interleukin 4; lipid peroxide; mitogenic agent; outer membrane protein; protein antibody; Pseudomonas exotoxin; Pseudomonas vaccine; recombinant protein; superoxide dismutase; thiobarbituric acid reactive substance; animal cell; animal experiment; animal model; animal tissue; antibody production; article; body surface; burn; controlled study; cytokine production; enzyme activity; Gram negative infection; immune response; immunization; male; mouse; nonhuman; priority journal; Pseudomonas aeruginosa; spleen cell; survival rate; vaccination; Bacteria (microorganisms); Pseudomonas; Pseudomonas aeruginosa |
顯示於: | 醫學系 |
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