https://scholars.lib.ntu.edu.tw/handle/123456789/477749
標題: | A Novel 96well-formatted Micro-gap Plate Enabling Drug Response Profiling on Primary Tumour Samples | 作者: | Ma, W.-Y. Hsiung, L.-C. Wang, C.-H. Chiang, C.-L. CHING-HUNG LIN CHIUN-SHENG HUANG ANDREW WO |
公開日期: | 2015 | 出版社: | Nature Publishing Group | 卷: | 5 | 起(迄)頁: | 9656 | 來源出版物: | Scientific Reports | 摘要: | Drug-based treatments are the most widely used interventions for cancer management. Personalized drug response profiling remains inherently challenging with low cell count harvested from tumour sample. We present a 96well-formatted microfluidic plate with built-in micro-gap that preserves up to 99.2% of cells during multiple assay/wash operation and only 9,000 cells needed for a single reagent test (i.e. 1,000 cells per test spot x 3 selected concentration x triplication), enabling drug screening and compatibility with conventional automated workstations. Results with MCF7 and MDA-MB-231 cell lines showed that no statistical significance was found in dose-response between the device and conventional 96-well plate control. Primary tumour samples from breast cancer patients tested in the device also showed good IC50 prediction. With drug screening of primary cancer cells must consider a wide range of scenarios, e.g. suspended/attached cell types and rare/abundant cell availability, the device enables high throughput screening even for suspended cells with low cell count since the signature microfluidic cell-trapping feature ensures cell preservation in a multiple solution exchange protocol. ? 2015, Nature Publishing Group. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84927623509&doi=10.1038%2fsrep09656&partnerID=40&md5=ff59f647c6056d6f4628e8ff643543a0 https://scholars.lib.ntu.edu.tw/handle/123456789/477749 |
ISSN: | 2045-2322 | DOI: | 10.1038/srep09656 | SDG/關鍵字: | antineoplastic agent; aged; Breast Neoplasms; dose response; drug resistance; female; human; in vitro study; middle aged; Neoplasms; pathology; preclinical study; procedures; tumor cell line; Aged; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Drug Resistance, Neoplasm; Female; Humans; In Vitro Techniques; Middle Aged; Neoplasms |
顯示於: | 醫學系 |
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