https://scholars.lib.ntu.edu.tw/handle/123456789/478364
標題: | Role of PGRMC1 in cell physiology of cervical cancer | 作者: | Shih C.-C. Chou H.-C. Chen Y.-J. WEN-HUNG KUO Chan C.-H. Lin Y.-C. Liao E.-C. Chang S.-J. Chan H.-L. |
關鍵字: | Cervical cancer; Metastasis; PGRMC1; Proteomics | 公開日期: | 2019 | 出版社: | Elsevier Inc. | 卷: | 231 | 起(迄)頁: | 116541 | 來源出版物: | Life Sciences | 摘要: | Aims: The most frequent cancers among women worldwide. The mortality of cervical cancer has declined significantly primarily due to the widespread use of Pap smear tests as a screening test and therapeutic vaccination. However, cervical cancer still remains a severe disease among the female population, as the prognosis of metastatic cervical cancer is very poor. Key methods: In this study, we performed 2D-DIGE and MALDI-TOF/TOF MS to analyze differentially expressed proteins between HeLa and invasive HeLa-I5 cells. Key findings: According to our proteomics data, 68 differentially expressed proteins between the HeLa and HeLa-I5 cells were identified. One of these differentially expressed proteins, Progesterone receptor membrane component 1 (PGRMC1), was selected as a candidate for further studies. To correlate the role of PGRMC1 with cellular migration and cancer progression, small interfering RNA (siRNA) was used to knockdown the expression of PGRMC1. Similar function of PGRMC1 was also observed in two other cervical cancer lines, CaSki and ME-180. Significance: PGRMC1 plays an essential role in regulating cancer progression and metastasis of cervical cancer cells, thus serving as a potential therapeutic target for cervical cancer. ? 2019 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067417367&doi=10.1016%2fj.lfs.2019.06.016&partnerID=40&md5=1f3683d67e7dd7ba9c71624ebb8350a8 https://scholars.lib.ntu.edu.tw/handle/123456789/478364 |
ISSN: | 0024-3205 | DOI: | 10.1016/j.lfs.2019.06.016 | SDG/關鍵字: | membrane protein; progesterone receptor membrane component 1; small interfering RNA; unclassified drug; membrane protein; PGRMC1 protein, human; progesterone receptor; Article; Ca Ski cell line; cancer growth; cell function; cell migration; cervical adenocarcinoma cell line; controlled study; female; gene knockdown; HeLa cell line; HeLa I5 cell line; human; human cell; human tissue; major clinical study; matrix assisted laser desorption ionization time of flight mass spectrometry; ME-180 cell line; metastasis; protein expression; protein function; proteomics; two dimensional gel electrophoresis; uterine cervix cancer; cell motion; cell proliferation; genetics; matrix-assisted laser desorption-ionization mass spectrometry; metabolism; pathology; physiology; procedures; tumor cell line; tumor invasion; two dimensional difference gel electrophoresis; uterine cervix tumor; Cell Line, Tumor; Cell Movement; Cell Proliferation; Female; HeLa Cells; Humans; Membrane Proteins; Neoplasm Invasiveness; Proteomics; Receptors, Progesterone; RNA, Small Interfering; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Two-Dimensional Difference Gel Electrophoresis; Uterine Cervical Neoplasms |
顯示於: | 醫學系 |
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