https://scholars.lib.ntu.edu.tw/handle/123456789/479320
標題: | Tumor-associated macrophages: The double-edged sword in cancer progression | 作者: | Chen J.J.W. Lin Y.-C. Yao P.-L. ANG YUAN Chen H.-Y. CHIA-TUNG SHUN Tsai M.-F. Chen C.-H. PAN-CHYR YANG |
公開日期: | 2005 | 卷: | 23 | 期: | 5 | 起(迄)頁: | 953-964 | 來源出版物: | Journal of Clinical Oncology | 摘要: | Purpose: Inflammation plays a critical role in cancer progression. In this study we investigate the pro-tumorigenic activities and gene expression profiles of lung cancer cells after interaction with macrophages. Materials and Methods: We measured intratumoral microvessel counts and macrophage density in 41 lung cancer tumor specimens and correlated these with the patients' clinical outcome. The interaction between macrophages and cancer cell lines was assessed using a transwell coculture system. The invasive potential was evaluated by in vitro invasion assay. The matrix-degrading activity was assayed by gelatin zymography. The microarray was applied to a large-scale analysis of the genes involved in the interaction, as well as to monitor the gene expression profiles of lung cancer cells responding to anti-inflammatory drugs in cocultures. Results: The macrophage density positively correlated with microvessel counts and negatively correlated with patient relapse-free survival (P < .05). After coculture with macrophages, lung cancer cell lines exhibited higher invasive potentials and matrix-degrading activities. We identified 50 genes by microarray that were upregulated more than two-fold in cancer cells after coculture. Northern blot analyses confirmed some gene expression such as interleukin-6, interleukin-8, and matrix metalloproteinase 9. The two-dimensional hierarchical clustering also demonstrated that the gene expression profiles of lung cancer cells responding to various anti-inflammatory drugs in cocultures are distinct. Conclusion: The interaction of lung cancer cells and macrophages can promote the invasiveness and matrix-degrading activity of cancer cells. Our results also suggest that a great diversity of gene expression occurs in this interaction, which may assist us in understanding the process of cancer metastasis. ? 2005 by American Society of Clinical Oncology. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/479320 | ISSN: | 0732-183X | DOI: | 10.1200/JCO.2005.12.172 | SDG/關鍵字: | acetylsalicylic acid; antiinflammatory agent; celecoxib; dexamethasone; gelatinase B; interleukin 6; interleukin 8; pyrrolidine dithiocarbamate; thalidomide; antiinflammatory agent; gelatinase; gelatinase B; interleukin 6; interleukin 8; adult; article; cancer growth; cancer invasion; cancer relapse; cancer survival; cell interaction; DNA microarray; female; gene expression; gene identification; human; human cell; lung cancer; macrophage; major clinical study; male; microarray analysis; Northern blotting; nucleotide sequence; priority journal; zymography; adenocarcinoma; cell count; coculture; disease course; disease free survival; drug effect; gene expression regulation; genetics; lung alveolus macrophage; lung non small cell cancer; lung tumor; microcirculation; middle aged; pathology; squamous cell carcinoma; tumor cell line; upregulation; vascularization; Adenocarcinoma; Anti-Inflammatory Agents; Carcinoma, Non-Small-Cell Lung; Carcinoma, Squamous Cell; Cell Count; Cell Line, Tumor; Coculture Techniques; Disease Progression; Disease-Free Survival; Female; Gelatinases; Gene Expression Regulation, Neoplastic; Humans; Interleukin-6; Interleukin-8; Lung Neoplasms; Macrophages, Alveolar; Male; Matrix Metalloproteinase 9; Microcirculation; Middle Aged; Neoplasm Invasiveness; Up-Regulation |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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