https://scholars.lib.ntu.edu.tw/handle/123456789/483284
Title: | Focused shockwave induced blood-brain barrier opening and transfection | Authors: | Kung Y. Lan C. MING-YEN HSIAO Sun M.-K. Hsu Y.-H. ABEL PO-HAO HUANG Liao W.-H. Liu H.-L. Inserra C. WEN-SHIANG CHEN HAO-LI LIU Y Kung C Lan MK Sun YH Hsu APH Liao WH Liao C Inserra WS Chen |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Journal Volume: | 8 | Journal Issue: | 1 | Start page/Pages: | 2218 | Source: | Scientific Reports | Abstract: | Despite extensive efforts in recent years, the blood-brain barrier (BBB) remains a significant obstacle for drug delivery. This study proposes using a clinical extracorporeal shockwave instrument to open the BBB, combined with a laser assisted bi-axial locating platform to achieve non-invasive, controllable-focus and reversible BBB opening in the brains of rats. Under shockwave treatment with an intensity level of 5 (P-9.79 MPa, energy flux density (EFD) 0.21 mJ/mm2) and a pulse repetition frequency of 5 Hz, the BBB could be opened after 50 shocks without the use of an ultrasound contrast agent. With the proposed method, the BBB opening can be precisely controlled in terms of depth, size and location. Moreover, a shockwave based gene transfection was demonstrated using a luciferase gene. © 2018 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041558908&doi=10.1038%2fs41598-018-20672-y&partnerID=40&md5=b1882cfc73afd554fdabee64c766d835 https://scholars.lib.ntu.edu.tw/handle/123456789/483284 |
ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-20672-y | SDG/Keyword: | luciferase; animal; blood brain barrier; brain; gene therapy; genetic transfection; genetics; high-energy shock wave; procedures; radiation response; rat; reporter gene; shock wave therapy; Animals; Blood-Brain Barrier; Brain; Extracorporeal Shockwave Therapy; Genes, Reporter; Genetic Therapy; High-Energy Shock Waves; Luciferases; Rats; Transfection |
Appears in Collections: | 醫學系 |
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