https://scholars.lib.ntu.edu.tw/handle/123456789/484558
標題: | Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system | 作者: | Huang, R.-X. Fu, Y. Liu, W. Ma, Y.-T. Hsieh, B.-Y. SHU-CHING CHEN Sun, M. Li, P.-C. PAI-CHI LI |
關鍵字: | 3D Cell Culture; Compressed Sensing; Matrix Completion; Photoacoustic Microscopy; Sparse Sampling | 公開日期: | 2018 | 卷: | 10494 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm3. To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs. ? COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/484558 | DOI: | 10.1117/12.2289975 | SDG/關鍵字: | Cell culture; Compressed sensing; Data acquisition; Diagnosis; Diseases; Gold compounds; Image processing; Irradiation; Matrix algebra; Optical systems; Photons; T-cells; Tumors; 3-D cell culture; Cytotoxic T lymphocytes; High spatial resolution; Low-rank matrix recoveries; Matrix completion; Nuclear norm minimizations; Optimization problems; Sparse sampling; Photoacoustic microscopy |
顯示於: | 生醫電子與資訊學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。