https://scholars.lib.ntu.edu.tw/handle/123456789/485849
標題: | Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array | 作者: | Kuo, C.-T. Wang, J.-Y. Lin, Y.-F. Wo, A.M. Chen, B.P.C. ANDREW WO H. Lee |
公開日期: | 2017 | 卷: | 7 | 期: | 1 | 來源出版物: | Scientific Reports | 摘要: | Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments. ? 2017 The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/485849 | DOI: | 10.1038/s41598-017-04718-1 | SDG/關鍵字: | biocompatible coated material; dimeticone; bioassay; cell culture technique; coculture; human; multicellular spheroid; preclinical study; procedures; tumor cell line; Biological Assay; Cell Culture Techniques; Cell Line, Tumor; Coated Materials, Biocompatible; Coculture Techniques; Dimethylpolysiloxanes; Drug Evaluation, Preclinical; Humans; Spheroids, Cellular |
顯示於: | 應用力學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。