https://scholars.lib.ntu.edu.tw/handle/123456789/486151
標題: | A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis | 作者: | Tsai, P.I. Lee, S.S. Chou, S.T. Jan, C.M. Chang, Y.T. Lee, A.S.Y. CHIH-KUNG LEE |
關鍵字: | Bithiophene; Ellipsometry; Interferon-gamma; Phase-interrogation; Surface plasmon resonance | 公開日期: | 2014 | 卷: | 571 | 期: | P3 | 起(迄)頁: | 473-477 | 來源出版物: | Thin Solid Films | 摘要: | Real-time, label-free monitoring offers a wide range of applications to the biotech field. We developed an integrated bio-sensing platform which adapts a circular polarization interferometry configuration and a phase modulated ellipsometer to improve the performance of biomolecular measurements. An bithiophene-based conductive linker-5′-(mercapto)-[2, 2′-bithiophene]-5-carboxylic acid was developed for detecting antibody-antigen interactions. In this study, the biomarker interferon-gamma (IFN-γ), one of the most important indicators of tuberculosis, was chosen to test and verify the sensitivity of our measurement system. Our experimental results showed that an increase in the concentration of the IFN-γ (64 pM to 1 μM) was usually accompanied by a phase increase. This result is good evidence that our biosensing system can be useful for analyzing biomolecular interactions. ? 2013 Elsevier B.V. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/486151 | DOI: | 10.1016/j.tsf.2013.12.037 | SDG/關鍵字: | Antigen-antibody reactions; Ellipsometry; Glycoproteins; Plasmons; Surface plasmon resonance; Antibody-antigen interactions; Biomolecular interactions; Biosensing systems; Bithiophenes; Interferon-gamma; Label-free monitoring; Phase-interrogation; Polarization interferometry; Antibiotics |
顯示於: | 應用力學研究所 |
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