https://scholars.lib.ntu.edu.tw/handle/123456789/491790
標題: | Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy | 作者: | Lee, C.-W. Chen, M.J. Cheng, J.-Y. Wei, P.-K. MIIN-JANG CHEN |
關鍵字: | image reconstruction; microscopy; scattering; surface plasmons | 公開日期: | 2009 | 卷: | 14 | 期: | 3 | 來源出版物: | Journal of Biomedical Optics | 摘要: | The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times. ? 2009 Society of Photo-Optical Instrumentation Engineers. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/491790 | DOI: | 10.1117/1.3147390 | SDG/關鍵字: | Cell morphology; Cytochalasin D; Dark-field; Electrostatic attractive forces; Focal Plane; Gold Nanoparticles; l-Lysine; Living cell; Lung cancer cells; Morphologic changes; Morphological images; Morphological study; Optical sections; Scattering intensity; surface plasmons; Amino acids; Drug interactions; Gold; Image reconstruction; Nanoparticles; Three dimensional; Bioinformatics; cytochalasin D; gold; metal nanoparticle; tumor necrosis factor alpha; algorithm; article; cell; cell culture; cell shape; chemistry; cytology; drug effect; HeLa cell; human; image processing; lung non small cell cancer; lung tumor; metabolism; methodology; microscopy; pathology; surface plasmon resonance; tissue distribution; tumor cell culture; Algorithms; Carcinoma, Non-Small-Cell Lung; Cell Shape; Cells; Cells, Cultured; Cytochalasin D; Gold; Hela Cells; Humans; Image Processing, Computer-Assisted; Lung Neoplasms; Metal Nanoparticles; Microscopy; Surface Plasmon Resonance; Tissue Distribution; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha |
顯示於: | 材料科學與工程學系 |
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