https://scholars.lib.ntu.edu.tw/handle/123456789/491790
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lee, C.-W. | en_US |
dc.contributor.author | Chen, M.J. | en_US |
dc.contributor.author | Cheng, J.-Y. | en_US |
dc.contributor.author | Wei, P.-K. | en_US |
dc.contributor.author | MIIN-JANG CHEN | en_US |
dc.creator | MIIN-JANG CHEN;Wei, P.-K.;Cheng, J.-Y.;Chen, M.J.;Lee, C.-W. | - |
dc.date.accessioned | 2020-05-12T02:52:27Z | - |
dc.date.available | 2020-05-12T02:52:27Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/491790 | - |
dc.description.abstract | The morphologic changes of living cells under drug interactions were studied by using 80-nm gold nanoparticles and dark-field optical section microscopy. The gold nanoparticles were coated with poly (L-lysine), which attached to the membranes of various cells by way of electrostatic attractive force. A three-dimensional (3-D) morphological image was obtained by measuring the peak scattering intensities of gold nanoparticles at different focal planes. An algorithm for the reconstruction of 3-D cell morphology was presented. With the measured nanoparticle images and calculations, we show morphologic changes of lung cancer cells under the interaction of cytochalasin D drug at different times. ? 2009 Society of Photo-Optical Instrumentation Engineers. | - |
dc.relation.ispartof | Journal of Biomedical Optics | - |
dc.subject | image reconstruction; microscopy; scattering; surface plasmons | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Cell morphology; Cytochalasin D; Dark-field; Electrostatic attractive forces; Focal Plane; Gold Nanoparticles; l-Lysine; Living cell; Lung cancer cells; Morphologic changes; Morphological images; Morphological study; Optical sections; Scattering intensity; surface plasmons; Amino acids; Drug interactions; Gold; Image reconstruction; Nanoparticles; Three dimensional; Bioinformatics; cytochalasin D; gold; metal nanoparticle; tumor necrosis factor alpha; algorithm; article; cell; cell culture; cell shape; chemistry; cytology; drug effect; HeLa cell; human; image processing; lung non small cell cancer; lung tumor; metabolism; methodology; microscopy; pathology; surface plasmon resonance; tissue distribution; tumor cell culture; Algorithms; Carcinoma, Non-Small-Cell Lung; Cell Shape; Cells; Cells, Cultured; Cytochalasin D; Gold; Hela Cells; Humans; Image Processing, Computer-Assisted; Lung Neoplasms; Metal Nanoparticles; Microscopy; Surface Plasmon Resonance; Tissue Distribution; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha | - |
dc.title | Morphological studies of living cells using gold nanoparticles and dark-field optical section microscopy | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1117/1.3147390 | - |
dc.identifier.scopus | 2-s2.0-70349257422 | - |
dc.identifier.url | https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349257422&doi=10.1117%2f1.3147390&partnerID=40&md5=6ddf7808363bfabba19898344d17b729 | - |
dc.relation.journalvolume | 14 | - |
dc.relation.journalissue | 3 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
crisitem.author.dept | Materials Science and Engineering | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 材料科學與工程學系 |
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