https://scholars.lib.ntu.edu.tw/handle/123456789/492385
標題: | A low cost color-based bacterial biosensor for measuring arsenic in groundwater | 作者: | Huang C.-W. CHIA-CHENG WEI Liao V.H.C. |
公開日期: | 2015 | 出版社: | Elsevier Ltd | 卷: | 141 | 起(迄)頁: | 44-49 | 來源出版物: | Chemosphere | 摘要: | Using arsenic (As) contaminated groundwater for drinking or irrigation has caused major health problems for humans around the world, raising a need to monitor As level efficiently and economically. This study developed a color-based bacterial biosensor which is easy-to-use and inexpensive for measuring As and could be complementary to current As detecting techniques. The arsR-lacZ recombinant gene cassette in nonpathogenic strain Escherichia coli DH5α was used in the color-based biosensor which could be observed by eyes or measured by spectrometer. The developed bacterial biosensor demonstrates a quantitative range from 10 to 500μgL-1 of As in 3-h reaction time. Furthermore, the biosensor was able to successfully detect and estimate As concentration in groundwater sample by measuring optical density at 595nm (OD595). Among different storage methods used in this study, biosensor in liquid at 4°C showed the longest shelf life about 9d, and liquid storage at RT and cell pellet could also be stored for about 3-5d. In conclusion, this study showed that the As biosensor with reliable color signal and economical preservation methods is useful for rapid screening of As pollutant, providing the potential for large scale screening and better management strategies for environmental quality control. ? 2015 Elsevier Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84949223563&doi=10.1016%2fj.chemosphere.2015.06.011&partnerID=40&md5=b82d588e41573bae3826f09c7b48209a https://scholars.lib.ntu.edu.tw/handle/123456789/492385 |
ISSN: | 0045-6535 | DOI: | 10.1016/j.chemosphere.2015.06.011 | SDG/關鍵字: | Arsenic; Color; Costs; Escherichia coli; Groundwater; Groundwater pollution; Arsenic in groundwater; Contaminated groundwater; Detecting technique; Environmental quality; Management strategies; Nonpathogenic strains; Preservation methods; Shelf life; Biosensors; arsenic; ground water; arsenic; ground water; water pollutant; arsenic; bioassay; color; detection method; groundwater pollution; quality control; arsR gene; Article; bacterial gene; bacterial strain; biosensor; color based biosensor; cost; Escherichia coli; lacZ gene; nonhuman; preservation; quantitative analysis; reaction time; recombinant gene; shelf life; water analysis; analysis; chemistry; color; colorimetry; cost benefit analysis; dose response; drug effects; economics; genetic procedures; genetics; plasmid; procedures; water pollutant; Bacteria (microorganisms); Escherichia coli; Arsenic; Biosensing Techniques; Color; Colorimetry; Cost-Benefit Analysis; Dose-Response Relationship, Drug; Escherichia coli; Groundwater; Plasmids; Water Pollutants, Chemical |
顯示於: | 食品安全與健康研究所 |
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