https://scholars.lib.ntu.edu.tw/handle/123456789/495794
標題: | A rapid method to study heat shock protein 70-2 gene polymorphism in insulin-dependent diabetes mellitus | 作者: | LEE-MING CHUANG TZUU-SHUH JOU HUEY-PEIR WU TONG-YUAN TAI Lin B.J. |
公開日期: | 1996 | 出版社: | Lippincott Williams and Wilkins | 卷: | 13 | 期: | 3 | 起(迄)頁: | 268-272 | 來源出版物: | Pancreas | 摘要: | To examine the role of DNA loci within the human leukocyte antigen (HLA) region and insulin-dependent diabetes mellitus (IDDM), we studied fine mapping of HSP70-2 gene. Polymerase chain reaction (PCR)-based genotyping was then developed and applied to type HSP70-2 in 59 patients with IDDM and 83 unrelated controls recruited from the inhabitants of northern Taiwan. Southern blot analysis revealed a diallelic PstI polymorphism of the HSP70-2 gene, i.e., 9.6- and 8.5-kb alleles. The polymorphic site was mapped in the intragenic PstI sequences (nucleotides 1051-1056) of the HSP70-2 gene. PCR- based restriction fragment length polymorphism studies revealed that the frequency of the 8.5-kb allele was increased in IDDM (56.8%, vs. 40.4% in controls; p < 0.009), with a relative risk of 1.93 (95% confidence interval 1.20-3.11). The genotypic frequencies of 9.6/9.6, 9.6/8.5, and 8.5/8.5 were 17.0, 52.5, and 30.5% for IDDM were different from those of controls (36.1, 47.0, and 16.9%, respectively; the homozygous 9.6/9.6 genotype was significantly decreased in the IDDM group, p < 0.02). In conclusion, we provide a simple, rapid, and nonradioactive method for HSP70-2 genotyping. Our data confirmed that the 8.5-kb allele of HSP70-2 was associated with IDDM susceptibility in the Taiwanese population. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029798718&doi=10.1097%2f00006676-199610000-00009&partnerID=40&md5=8904a7e7c3bf22b59c500e02cf478cce https://scholars.lib.ntu.edu.tw/handle/123456789/495794 |
ISSN: | 0885-3177 | DOI: | 10.1097/00006676-199610000-00009 | SDG/關鍵字: | heat shock protein; adult; article; gene mapping; genetic polymorphism; genetic risk; genotype; human; human cell; insulin dependent diabetes mellitus; major clinical study; polymerase chain reaction; priority journal; restriction fragment length polymorphism; Southern blotting; technique |
顯示於: | 醫學系 |
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