https://scholars.lib.ntu.edu.tw/handle/123456789/496158
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wang S.-Y. | en_US |
dc.contributor.author | Tseng C.-P. | en_US |
dc.contributor.author | Tsai K.-C. | en_US |
dc.contributor.author | Lin C.-F. | en_US |
dc.contributor.author | Wen C.-Y. | en_US |
dc.contributor.author | Tsay H.-S. | en_US |
dc.contributor.author | Sakamoto N. | en_US |
dc.contributor.author | CHIN-HSIAO TSENG | en_US |
dc.contributor.author | Cheng J.-C. | en_US |
dc.creator | Wang S.-Y.;Tseng C.-P.;Tsai K.-C.;Lin C.-F.;Wen C.-Y.;Tsay H.-S.;Sakamoto N.;Chin-Hsiao Tseng;Cheng J.-C. | - |
dc.date.accessioned | 2020-06-01T08:33:49Z | - |
dc.date.available | 2020-06-01T08:33:49Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-67349091694&doi=10.1016%2fj.bbrc.2009.05.043&partnerID=40&md5=e8803187fcbf5ed48767725fe5dcbd38 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/496158 | - |
dc.description.abstract | Chronic hepatitis C virus (HCV) infection is a worldwide public issue. In this study, we performed bioactivity-guided screening of the Lonicera hypoglauca Miq. crude extracts to find for naturally chemical entities with anti-HCV activity. Pheophytin a was identified from the ethanol-soluble fraction of L. hypoglauca that elicited dose-dependent inhibition of HCV viral proteins and RNA expression in both replicon cells and cell culture infectious system. Computational modeling revealed that pheophytin a can bind to the active site of HCV-NS3, suggesting that NS3 is a potent molecular target of pheophytin a. Biochemical analysis further revealed that pheophytin a inhibited NS3 serine protease activity with IC50 = 0.89 μM. Notably, pheophytin a and IFNα-2a elicited synergistic anti-HCV activity in replicon cells with no significant cytotoxicity. This study thereby demonstrates for the first time that pheophytin a is a potent HCV-NS3 protease inhibitor and offers insight for development of novel anti-HCV regimens. ? 2009 Elsevier Inc. All rights reserved. | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | alpha2a interferon; antivirus agent; nonstructural protein 3; pheophytin a; plant extract; serine proteinase; unclassified drug; virus protein; virus RNA; antiviral activity; article; cell culture; cell survival; cell viability; concentration response; controlled study; drug cytotoxicity; drug potency; drug potentiation; drug protein binding; drug screening; enzyme inhibition; gene expression; Hepatitis C virus; human; human cell; hydrogen bond; IC 50; Lonicera hypoglauca; medicinal plant; molecular model; nonhuman; plant leaf; plant stem; priority journal; protein expression; replicon; Antiviral Agents; Catalytic Domain; Cell Line; Computer Simulation; Drug Evaluation, Preclinical; Hepacivirus; Humans; Interferon Alfa-2a; Lonicera; Models, Molecular; Pheophytins; Viral Nonstructural Proteins; Viral Proteins; Virus Replication; Hepatitis C virus; Lonicera | - |
dc.title | Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq. | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.bbrc.2009.05.043 | - |
dc.identifier.pmid | 19450556 | - |
dc.identifier.scopus | 2-s2.0-67349091694 | - |
dc.relation.pages | 230-235 | - |
dc.relation.journalvolume | 385 | - |
dc.relation.journalissue | 2 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0001-9545-7123 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。