https://scholars.lib.ntu.edu.tw/handle/123456789/502943
Title: | Expression and antigenicity of human immunodeficiency virus type-1 transmembrane protein GP41 in insect cells | Authors: | SUNG-LIANG YU Chou M.-J. Tam M.F. Lee T.-H. Syu W.-J. |
Issue Date: | 1993 | Journal Volume: | 191 | Journal Issue: | 1 | Start page/Pages: | 207-213 | Source: | Biochemical and Biophysical Research Communications | Abstract: | The HIV-1 transmembrane protein, gp41, is processed together with the envelope glycoprotein, gp120, from the same precursor, gp160, during the virus maturation. We used a baculovirus expression system to demonstrate that gp41 could be properly expressed without the preceding gp120 sequence. Two constructs with slight differences in the N-terminal region of gp41 were generated: one with a deletion of the first 7 hydrophobic residues of gp41, which have been suggested to be in a region important for membrane fusion and penetration, whereas the second with a complete sequence of gp41 except that a nonconserved leucine was substituted with a glutamine during DNA manipulation. Results from Western blotting with specific antisera confirm the gp41 identity. The sizes of gp41 were sensitive to tunicamycin treatment, indicating that N-linked glycosylation did occur. Further immunoblotting analyses with 90 different serum samples from HIV-1 -infected individuals gave similar reaction patterns, suggesting that gp120 as well as the N-terminal region of gp4l are not critical for the expression and antigenecity of gp41. These eucaryotic constructs should provide valuable gp41 sources for detailed characterization of gp41 functions. ? 1993 Academic Press. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/502943 | ISSN: | 0006-291X | DOI: | 10.1006/bbrc.1993.1204 | SDG/Keyword: | glycoprotein; membrane protein; amino acid sequence; animal cell; article; cell membrane; gene expression; human immunodeficiency virus; immunoblotting; insect; nonhuman; nucleotide sequence; priority journal; Amino Acid Sequence; Animal; Baculoviridae; Base Sequence; Blotting, Western; Cell Line; Epitopes; Gene Expression; Genetic Vectors; HIV Envelope Protein gp120; HIV Envelope Protein gp41; HIV-1; Insects; Molecular Sequence Data; Mutagenesis, Insertional; Oligodeoxyribonucleotides; Plasmids; Restriction Mapping; Support, Non-U.S. Gov't; Transfection; Tunicamycin |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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