https://scholars.lib.ntu.edu.tw/handle/123456789/502943
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | SUNG-LIANG YU | en_US |
dc.contributor.author | Chou M.-J. | en_US |
dc.contributor.author | Tam M.F. | en_US |
dc.contributor.author | Lee T.-H. | en_US |
dc.contributor.author | Syu W.-J. | en_US |
dc.creator | Sung-Liang Yu;Chou M.-J.;Tam M.F.;Lee T.-H.;Syu W.-J. | - |
dc.date.accessioned | 2020-06-17T03:02:04Z | - |
dc.date.available | 2020-06-17T03:02:04Z | - |
dc.date.issued | 1993 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/502943 | - |
dc.description.abstract | The HIV-1 transmembrane protein, gp41, is processed together with the envelope glycoprotein, gp120, from the same precursor, gp160, during the virus maturation. We used a baculovirus expression system to demonstrate that gp41 could be properly expressed without the preceding gp120 sequence. Two constructs with slight differences in the N-terminal region of gp41 were generated: one with a deletion of the first 7 hydrophobic residues of gp41, which have been suggested to be in a region important for membrane fusion and penetration, whereas the second with a complete sequence of gp41 except that a nonconserved leucine was substituted with a glutamine during DNA manipulation. Results from Western blotting with specific antisera confirm the gp41 identity. The sizes of gp41 were sensitive to tunicamycin treatment, indicating that N-linked glycosylation did occur. Further immunoblotting analyses with 90 different serum samples from HIV-1 -infected individuals gave similar reaction patterns, suggesting that gp120 as well as the N-terminal region of gp4l are not critical for the expression and antigenecity of gp41. These eucaryotic constructs should provide valuable gp41 sources for detailed characterization of gp41 functions. ? 1993 Academic Press. | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | glycoprotein; membrane protein; amino acid sequence; animal cell; article; cell membrane; gene expression; human immunodeficiency virus; immunoblotting; insect; nonhuman; nucleotide sequence; priority journal; Amino Acid Sequence; Animal; Baculoviridae; Base Sequence; Blotting, Western; Cell Line; Epitopes; Gene Expression; Genetic Vectors; HIV Envelope Protein gp120; HIV Envelope Protein gp41; HIV-1; Insects; Molecular Sequence Data; Mutagenesis, Insertional; Oligodeoxyribonucleotides; Plasmids; Restriction Mapping; Support, Non-U.S. Gov't; Transfection; Tunicamycin | - |
dc.title | Expression and antigenicity of human immunodeficiency virus type-1 transmembrane protein GP41 in insect cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1006/bbrc.1993.1204 | - |
dc.identifier.pmid | 7680556 | - |
dc.identifier.scopus | 2-s2.0-0027202671 | - |
dc.relation.pages | 207-213 | - |
dc.relation.journalvolume | 191 | - |
dc.relation.journalissue | 1 | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Clinical Laboratory Sciences and Medical Biotechnology | - |
crisitem.author.dept | Pathology | - |
crisitem.author.dept | Medical Device and Imaging | - |
crisitem.author.orcid | 0000-0003-4535-9036 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學檢驗暨生物技術學系 |
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